SPAG4 sirna

SPAG4 siRNA (Human)

Cat. Num. AAA8237917

• Gene Names: SPAG4; SUN4; CT127
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPAG4; SPAG4 siRNA (Human); SUN4; Sperm-associated antigen 4 protein; Outer dense fiber-associated protein SPAG4; SUN domain-containing protein 4; SPAG4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPAG4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 437

• Applicable Applications for SPAG4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPAG4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPAG4 sirna

siRNA to inhibit SPAG4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11037055
• NCBI GeneID: 6676
• NCBI Accession #: NP_003107.1
• NCBI GenBank Nucleotide #: NM_003116.1
• UniProt Accession #: Q9NPE6; O43648
• Molecular Weight: 48,165 Da
• NCBI Official Full Name: sperm-associated antigen 4 protein
• NCBI Official Synonym Full Names: sperm associated antigen 4
• NCBI Official Symbol: SPAG4
• NCBI Official Synonym Symbols: SUN4; CT127
• NCBI Protein Information: sperm-associated antigen 4 protein
• UniProt Protein Name: Sperm-associated antigen 4 protein
• Protein Family: Sperm-associated antigen 4 protein
• UniProt Gene Name: SPAG4
• UniProt Synonym Gene Names: SUN4
• UniProt Entry Name: SPAG4_HUMAN

NCBI Description

The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. [provided by RefSeq, Jul 2008]

Uniprot Description

SPAG4: May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.

Protein type: Cancer Testis Antigen (CTA); Microtubule-binding; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: cytoskeleton; cytoplasm; integral to membrane; nuclear envelope

Molecular Function: structural molecule activity

Biological Process: spermatogenesis; nuclear membrane organization and biogenesis