SOS1 sirna

SOS1 siRNA (Human)

Cat. Num. AAA8204857

• Gene Names: SOS1; GF1; HGF; NS4; GGF1; GINGF
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SOS1; SOS1 siRNA (Human); Son of sevenless homolog 1; SOS-1; SOS1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SOS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1333

• Applicable Applications for SOS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SOS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SOS1 sirna

siRNA to inhibit SOS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15529996
• NCBI GeneID: 6654
• NCBI Accession #: NP_005624.2
• NCBI GenBank Nucleotide #: NM_005633.3
• UniProt Accession #: Q07889; A8K2G3; B4DXG2
• Molecular Weight: 36,073 Da
• NCBI Official Full Name: son of sevenless homolog 1
• NCBI Official Synonym Full Names: son of sevenless homolog 1 (Drosophila)
• NCBI Official Symbol: SOS1
• NCBI Official Synonym Symbols: GF1; HGF; NS4; GGF1; GINGF
• NCBI Protein Information: son of sevenless homolog 1
• UniProt Protein Name: Son of sevenless homolog 1
• Protein Family: Son of sevenless
• UniProt Gene Name: SOS1
• UniProt Synonym Gene Names: SOS-1
• UniProt Entry Name: SOS1_HUMAN

NCBI Description

This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008]

Uniprot Description

SOS1: a guanine nucleotide exchange factor that activates Ras by catalyzing the exchange of bound GDP for GTP. Interacts with GRB2, NCK, and other adapter proteins. Plays an important role in signaling pathways leading from the G-protein- coupled receptors and receptor tyrosine kinases to MAPK/ERK.

Protein type: GEFs; Adaptor/scaffold; Motility/polarity/chemotaxis; GEFs, Ras

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cell soma; postsynaptic density; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; DNA binding; protein heterodimerization activity; SH3 domain binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; signal transduction; positive regulation of small GTPase mediated signal transduction; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of epidermal growth factor receptor signaling pathway; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; blood coagulation; leukocyte migration

Disease: Fibromatosis, Gingival, 1; Noonan Syndrome 4