SOCS7 sirna

SOCS7 siRNA (Human)

Cat. Num. AAA8213164

• Gene Names: SOCS7; NAP4; NCKAP4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SOCS7; SOCS7 siRNA (Human); NAP4; SOCS6; Suppressor of cytokine signaling 7; SOCS-7; Nck. Ash and phospholipase C gamma-binding protein; Nck-associated protein 4; NAP-4; SOCS7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SOCS7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 581

• Applicable Applications for SOCS7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SOCS7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SOCS7 sirna

siRNA to inhibit SOCS7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 53831997
• NCBI GeneID: 30837
• NCBI Accession #: NP_055413.1
• NCBI GenBank Nucleotide #: NM_014598.3
• UniProt Accession #: O14512; Q0IJ63; A2VCU2
• Molecular Weight: 37,461 Da
• NCBI Official Full Name: suppressor of cytokine signaling 7
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 7
• NCBI Official Symbol: SOCS7
• NCBI Official Synonym Symbols: NAP4; NCKAP4
• NCBI Protein Information: suppressor of cytokine signaling 7
• UniProt Protein Name: Suppressor of cytokine signaling 7
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS7
• UniProt Synonym Gene Names: NAP4; SOCS6; SOCS-7; NAP-4
• UniProt Entry Name: SOCS7_HUMAN

Uniprot Description

SOCS7: Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF- like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleolus; plasma membrane; nucleus

Molecular Function: protein binding; protein kinase inhibitor activity; SH3 domain binding

Biological Process: fat cell differentiation; regulation of growth; cytokine and chemokine mediated signaling pathway; insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; negative regulation of JAK-STAT cascade