SNTB2 sirna

SNTB2 siRNA (Human)

Cat. Num. AAA8213288

• Gene Names: SNTB2; SNT3; SNTL; SNT2B2; EST25263; D16S2531E
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNTB2; SNTB2 siRNA (Human); D16S2531E; SNT2B2; SNTL; Beta-2-syntrophin; 59 kDa dystrophin-associated protein A1 basic component 2; Syntrophin-3; SNT3; Syntrophin-like; SNTB2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SNTB2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 540

• Applicable Applications for SNTB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SNTB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNTB2 sirna

siRNA to inhibit SNTB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5803177
• NCBI GeneID: 6645
• NCBI Accession #: NP_006741.1
• NCBI GenBank Nucleotide #: NM_006750.3
• UniProt Accession #: Q13425; Q9BY09
• Molecular Weight: 27,677 Da
• NCBI Official Full Name: beta-2-syntrophin
• NCBI Official Synonym Full Names: syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2)
• NCBI Official Symbol: SNTB2
• NCBI Official Synonym Symbols: SNT3; SNTL; SNT2B2; EST25263; D16S2531E
• NCBI Protein Information: beta-2-syntrophin
• UniProt Protein Name: Beta-2-syntrophin
• Protein Family: Beta-2-syntrophin
• UniProt Gene Name: SNTB2
• UniProt Synonym Gene Names: D16S2531E; SNT2B2; SNTL; SNT3; SNTL
• UniProt Entry Name: SNTB2_HUMAN

NCBI Description

Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes. [provided by RefSeq, Jul 2008]

Uniprot Description

SNTB2: Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. May play a role in the regulation of secretory granules via its interaction with PTPRN. Monomer and homodimer (Probable). Interacts with the other members of the syntrophin family: SNTA1 and SNTB1; and with the sodium channel proteins SCN4A and SCN5A. Interacts with SAST, MAST205, microtubules and microtubule-associated proteins. Interacts with the dystrophin protein DMD and related proteins DTNA and UTRN, and with the neuroregulin receptor ERBB4. Interacts with PTPRN when phosphorylated, protecting PTPRN from protein cleavage by CAPN1. Dephosphorylation upon insulin stimulation disrupts the interaction with PTPRN and results in the cleavage of PTPRN. Ubiquitous. Isoform 1 is the predominant isoform. Weak level of isoform 2 is present in all tested tissues, except in liver and heart where it is highly expressed. Belongs to the syntrophin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Dystrophin complex; Adaptor/scaffold; Extracellular matrix

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: dystrophin-associated glycoprotein complex; microtubule; protein complex; focal adhesion; membrane; cytoplasm; synapse

Molecular Function: calmodulin binding; protein binding; actin binding