Immunohistochemistry (IHC)
(Immunochemical staining of human ALPI in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Positive staining was localized to hepatocyte.)
Rabbit anti-Human Alkaline phosphatase Polyclonal Antibody | anti-ALPI antibody
Anti-Alkaline phosphatase Antibody, Rabbit Polyclonal
Gene Names
ALPI; IAP
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Alkaline phosphatase; Polyclonal Antibody; Anti-Alkaline phosphatase Antibody; Rabbit Polyclonal; Alkaline Phosphatase/ALPI Antibody; Rabbit PAb; Antigen Affinity Purified; alkaline phosphatase; intestinal; Anti-IAP Antibody; anti-ALPI antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ALPI/IAP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ALPI antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Human ALPI/IAP protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human ALPI/IAP (rh ALPI/IAP). ALPI/IAP specific IgG was purified by Human ALPI/IAP affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human ALPI in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Positive staining was localized to hepatocyte.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ALPI in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Positive staining was localized to hepatocyte.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ALPI in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Positive staining was localized to nephric tubule.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ALPI in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Positive staining was localized to nephric tubule.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ALPI in human esophagus with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Positive staining was localized to epithelium.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ALPI in human esophagus with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Positive staining was localized to epithelium.)
Related Product Information for anti-ALPI antibody
ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Product Categories/Family for anti-ALPI antibody
References
Nakano T, et al. (2009) Role of lysophosphatidylcholine in brush-border intestinal alkaline phosphatase release and restoration. Am J Physiol Gastrointest Liver Physiol. 297(1): G207-14.Henthorn PS, et al. (1987) Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase. Proc Natl Acad Sci. 84(5): 1234-8.Oetting WS, et al. (2003) Oculocutaneous albinism type 1: the last 100 years. Pigment Cell Res. 16(3): 307-11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,812 Da
NCBI Official Full Name
intestinal-type alkaline phosphatase
NCBI Official Synonym Full Names
alkaline phosphatase, intestinal
NCBI Official Symbol
ALPI
NCBI Official Synonym Symbols
IAP
NCBI Protein Information
intestinal-type alkaline phosphatase; Kasahara isozyme; alkaline phosphomonoesterase; glycerophosphatase; intestinal alkaline phosphatase
UniProt Protein Name
Intestinal-type alkaline phosphatase
Protein Family
UniProt Gene Name
ALPI
UniProt Entry Name
PPBI_HUMAN
NCBI Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]
Research Articles on ALPI
Similar Products
Product Notes
The ALPI alpi (Catalog #AAA8106857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alkaline phosphatase Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline phosphatase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the ALPI alpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alkaline phosphatase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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