SNF8 sirna

SNF8 siRNA (Human)

Cat. Num. AAA8234973

• Gene Names: SNF8; Dot3; EAP30; VPS22
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNF8; SNF8 siRNA (Human); EAP30; Vacuolar-sorting protein SNF8; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22; SNF8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SNF8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 258

• Applicable Applications for SNF8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SNF8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNF8 sirna

siRNA to inhibit SNF8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21361380
• NCBI GeneID: 11267
• NCBI Accession #: NP_009172.2
• NCBI GenBank Nucleotide #: NM_007241.2
• UniProt Accession #: Q96H20; Q8IXY3; Q9UN50
• Molecular Weight: 28,736 Da
• NCBI Official Full Name: vacuolar-sorting protein SNF8
• NCBI Official Synonym Full Names: SNF8, ESCRT-II complex subunit
• NCBI Official Symbol: SNF8
• NCBI Official Synonym Symbols: Dot3; EAP30; VPS22
• NCBI Protein Information: vacuolar-sorting protein SNF8
• UniProt Protein Name: Vacuolar-sorting protein SNF8
• Protein Family: Vacuolar-sorting protein
• UniProt Gene Name: SNF8
• UniProt Synonym Gene Names: EAP30; hVps22
• UniProt Entry Name: SNF8_HUMAN

NCBI Description

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM, Mar 2008]

Uniprot Description

SNF8: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Belongs to the SNF8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; transcription factor complex; late endosome membrane; cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport