Rabbit anti-Human SNF8 Polyclonal Antibody | anti-SNF8 antibody

SNF8, NT (SNF8, EAP30, Vacuolar-sorting protein SNF8, ELL-associated protein of 30kD, ESCRT-II complex subunit VPS22) (AP)

Cat. Num. AAA6349309

• Gene Names: SNF8; Dot3; EAP30; VPS22
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNF8; Polyclonal Antibody; NT (SNF8; EAP30; Vacuolar-sorting protein SNF8; ELL-associated protein of 30kD; ESCRT-II complex subunit VPS22) (AP); ESCRT-II complex subunit VPS22; anti-SNF8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SNF8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human SNF8.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SNF8 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SNF8 antibody detected the SNF8 protein (arrow).)

Western Blot (WB)

(Western blot analysis of SNF8 (arrow) using rabbit polyclonal SNF8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.)

Related Product Information for anti-SNF8 antibody

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).

Product Categories/Family for anti-SNF8 antibody

Antibodies; Proteins

References

Malerod, L., et al. Traffic 8(11):1617-1629(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Langelier, C., et al. J. Virol. 80(19):9465-9480(2006). Bowers, K., et al. J. Biol. Chem. 281(8):5094-5105(2006). Slagsvold, T., et al. J. Biol. Chem. 280(20):19600-19606(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 21361380
• NCBI GeneID: 11267
• NCBI Accession #: NP_009172.2
• NCBI GenBank Nucleotide #: NM_007241.2
• UniProt Accession #: Q96H20; Q8IXY3; Q9UN50
• Molecular Weight: 28,736 Da
• NCBI Official Full Name: vacuolar-sorting protein SNF8
• NCBI Official Synonym Full Names: SNF8, ESCRT-II complex subunit
• NCBI Official Symbol: SNF8
• NCBI Official Synonym Symbols: Dot3; EAP30; VPS22
• NCBI Protein Information: vacuolar-sorting protein SNF8; EAP30 subunit of ELL complex; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; SNF8, ESCRT-II complex subunit, homolog; hVps22
• UniProt Protein Name: Vacuolar-sorting protein SNF8
• Protein Family: Vacuolar-sorting protein
• UniProt Gene Name: SNF8
• UniProt Synonym Gene Names: EAP30; hVps22
• UniProt Entry Name: SNF8_HUMAN

NCBI Description

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM, Mar 2008]

Uniprot Description

SNF8: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Belongs to the SNF8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; transcription factor complex; late endosome membrane; cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport