SLC6A3 sirna

SLC6A3 siRNA (Human)

Cat. Num. AAA8213973

• Gene Names: SLC6A3; DAT; DAT1; PKDYS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC6A3; SLC6A3 siRNA (Human); DAT1; Sodium-dependent dopamine transporter; DA transporter; DAT; Solute carrier family 6 member 3; SLC6A3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC6A3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 620

• Applicable Applications for SLC6A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC6A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC6A3 sirna

siRNA to inhibit SLC6A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4507041
• NCBI GeneID: 6531
• NCBI Accession #: NP_001035.1
• NCBI GenBank Nucleotide #: NM_001044.4
• UniProt Accession #: Q01959; Q14996; A2RUN4
• Molecular Weight: 68,495 Da
• NCBI Official Full Name: sodium-dependent dopamine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 3
• NCBI Official Symbol: SLC6A3
• NCBI Official Synonym Symbols: DAT; DAT1; PKDYS
• NCBI Protein Information: sodium-dependent dopamine transporter
• UniProt Protein Name: Sodium-dependent dopamine transporter
• UniProt Gene Name: SLC6A3
• UniProt Synonym Gene Names: DAT1; DA transporter; DAT
• UniProt Entry Name: SC6A3_HUMAN

NCBI Description

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

Uniprot Description

DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: cell soma; axon; integral to plasma membrane; cytoplasm; plasma membrane; integral to membrane

Molecular Function: protein binding; protease binding; dopamine binding; protein complex binding; monoamine transmembrane transporter activity; protein N-terminus binding; dopamine:sodium symporter activity; drug binding; dopamine transmembrane transporter activity; receptor binding

Biological Process: response to nicotine; lactation; response to drug; dopamine transport; response to cAMP; dopamine catabolic process; prepulse inhibition; monoamine transport; sensory perception of smell; regulation of dopamine metabolic process; locomotory behavior; positive regulation of multicellular organism growth; dopamine biosynthetic process; response to cocaine; synaptic transmission; response to ethanol; neurotransmitter transport; adenohypophysis development; response to iron ion; transmembrane transport; neurotransmitter biosynthetic process; aging

Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To