SLC27A4 sirna

SLC27A4 siRNA (Human)

Cat. Num. AAA8232376

• Gene Names: SLC27A4; IPS; FATP4; ACSVL4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC27A4; SLC27A4 siRNA (Human); ACSVL4; FATP4; Long-chain fatty acid transport protein 4; FATP-4; Fatty acid transport protein 4; Solute carrier family 27 member 4; SLC27A4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SLC27A4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 643

• Applicable Applications for SLC27A4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC27A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC27A4 sirna

siRNA to inhibit SLC27A4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40807357
• NCBI GeneID: 10999
• NCBI Accession #: NP_005085.2
• NCBI GenBank Nucleotide #: NM_005094.3
• UniProt Accession #: Q6P1M0; O95186; Q96G53; A8K2F7
• Molecular Weight: 26,001 Da
• NCBI Official Full Name: long-chain fatty acid transport protein 4
• NCBI Official Synonym Full Names: solute carrier family 27 (fatty acid transporter), member 4
• NCBI Official Symbol: SLC27A4
• NCBI Official Synonym Symbols: IPS; FATP4; ACSVL4
• NCBI Protein Information: long-chain fatty acid transport protein 4
• UniProt Protein Name: Long-chain fatty acid transport protein 4
• Protein Family: Long-chain fatty acid transport protein
• UniProt Gene Name: SLC27A4
• UniProt Synonym Gene Names: ACSVL4; FATP4; FATP-4; Fatty acid transport protein 4
• UniProt Entry Name: S27A4_HUMAN

NCBI Description

This gene encodes a member of a family of fatty acid transport proteins, which are involved in translocation of long-chain fatty acids cross the plasma membrane. This protein is expressed at high levels on the apical side of mature enterocytes in the small intestine, and appears to be the principal fatty acid transporter in enterocytes. Clinical studies suggest this gene as a candidate gene for the insulin resistance syndrome. Mutations in this gene have been associated with ichthyosis prematurity syndrome. [provided by RefSeq, Apr 2010]

Uniprot Description

SLC27A4: Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. Defects in SLC27A4 are the cause of ichthyosis prematurity syndrome (IPS). A keratinization disorder characterized by complications in the second trimester of pregnancy resulting from polyhydramnion, with premature birth of a child with thick caseous desquamating epidermis, respiratory complications and transient eosinophilia. After recovery during the first months of life, the symptoms are relatively benign and the patients suffer from a lifelong non-scaly ichthyosis with atopic manifestations. Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: Membrane protein, multi-pass; EC 6.2.1.-; Membrane protein, integral; Transporter, SLC family; Ligase; Transporter

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: endoplasmic reticulum membrane; microvillus; membrane; brush border membrane; plasma membrane; integral to membrane

Molecular Function: fatty acid transporter activity; nucleotide binding; very-long-chain-fatty-acid-CoA ligase activity; long-chain-fatty-acid-CoA ligase activity

Biological Process: long-chain fatty acid transport; skin development; very-long-chain fatty acid catabolic process; transport; lipid metabolic process; long-chain fatty acid metabolic process; medium-chain fatty acid transport; transmembrane transport; fatty acid transport; response to nutrient

Disease: Ichthyosis Prematurity Syndrome