Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit | UGT1A1 elisa kit

Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit

Cat. Num. AAA2706266

• Gene Names: UGT1A1; GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1
• Reactivity: Human
• Synonyms: UDP Glucuronosyltransferase 1 Family; Polypeptide A1 (UGT1A1); Human UDP Glucuronosyltransferase 1 Family; Polypeptide A1 (UGT1A1) ELISA Kit; UGT1; GNT1; UGT1A; UDPGT; HUG-BR1; UGT1-1; Bilirubin-specific UDPGT isozyme 1; UDP-glucuronosyltransferase 1-1; UGT1A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1).; No significant cross-reactivity or interference between UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) and analogues was observed.
• Sequence Length: 444

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.59ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UGT1A1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 118763983
• NCBI GeneID: 54658
• UniProt Accession #: P22309; A6NJC3; B8K286
• Molecular Weight: 49,368 Da
• NCBI Official Full Name: UDP glucuronosyltransferase 1 family, polypeptide A1
• NCBI Official Synonym Full Names: UDP glucuronosyltransferase family 1 member A1
• NCBI Official Symbol: UGT1A1
• NCBI Official Synonym Symbols: GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1
• NCBI Protein Information: UDP-glucuronosyltransferase 1-1
• UniProt Protein Name: UDP-glucuronosyltransferase 1-1
• Protein Family: UDP-glucuronosyltransferase
• UniProt Gene Name: UGT1A1
• UniProt Synonym Gene Names: GNT1; UGT1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; hUG-BR1; UGT-1A; UGT1A

NCBI Description

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX-alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4-methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.