SEPT9 sirna

SEPT9 siRNA (Human)

Cat. Num. AAA8240114

• Gene Names: SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SEPT9; SEPT9 siRNA (Human); KIAA0991; MSF; Septin-9; MLL septin-like fusion protein MSF-A; MLL septin-like fusion protein; Ovarian/Breast septin; Ov/Br septin; Septin D1; SEPT9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SEPT9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 586

• Applicable Applications for SEPT9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SEPT9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SEPT9 sirna

siRNA to inhibit SEPT9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 164698494
• NCBI GeneID: 10801
• NCBI Accession #: NP_001106963.1
• NCBI GenBank Nucleotide #: NM_001113491.1
• UniProt Accession #: Q9UHD8; Q96QF3; Q96QF4; Q96QF5; Q9HA04; Q9UG40; A8K2V3; B3KPM0; B4DTL9; B4E0N2; B4E274; B7Z654
• Molecular Weight: 41,187 Da
• NCBI Official Full Name: septin-9 isoform a
• NCBI Official Synonym Full Names: septin 9
• NCBI Official Symbol: SEPT9
• NCBI Official Synonym Symbols: MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
• NCBI Protein Information: septin-9
• UniProt Protein Name: Septin-9
• UniProt Gene Name: SEPT9
• UniProt Synonym Gene Names: KIAA0991; MSF; MLL septin-like fusion protein; Ov/Br septin
• UniProt Entry Name: SEPT9_HUMAN

NCBI Description

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009]

Uniprot Description

SEPT9: a member of a family of novel GTP binding proteins that have been implicated in cytokinesis and exocytosis. This family include the cell division control proteins CDC3, CDC10, CDC11. Required for the completion of cytokinesis and suggest a role that is distinct from that of Nedd5.

Protein type: Hydrolase; Cell cycle regulation; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: microtubule; perinuclear region of cytoplasm; cytoplasm; stress fiber; actin cytoskeleton

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: protein heterooligomerization; metabolic process; cell division; cell cycle

Disease: Amyotrophy, Hereditary Neuralgic