Rabbit KDM2B Polyclonal Antibody | anti-KDM2B antibody

KDM2B Antibody

Cat. Num. AAA9611302

• Gene Names: KDM2B; CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
• Reactivity: Human; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KDM2B; Polyclonal Antibody; KDM2B Antibody; [Histone-H3]-lysine-36 demethylase 1B; CXXC-type zinc finger protein 2; CXXC2; F box and leucine rich repeat protein 10; F box protein FBL10; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; Fbl10; FBXL10; JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein; JHDM1B; JmjC domain-containing histone demethylation protein 1B; Jumonji C domain containing histone demethylase 1B; Jumonji domain-containing EMSY-interactor methyltransferase motif protein; kdm2b; KDM2B_HUMAN; Lysine (K) specific demethylase 2B; Lysine-specific demethylase 2B; PCCX2; Protein containing CXXC domain 2; Protein JEMMA; Protein-containing CXXC domain 2; anti-KDM2B antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KDM2B Antibody detects endogenous levels of KDM2B.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1265

• Applicable Applications for anti-KDM2B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human KDM2B.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using KDM2B Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-KDM2B antibody

Description: Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Subunit Structure: Directly interacts with SKP1 and CUL1.

Similarity: The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes. Belongs to the JHDM1 histone demethylase family.

NCBI and Uniprot Product Information

• NCBI GI #: 54112380
• NCBI GeneID: 84678
• NCBI Accession #: NP_001005366.1
• NCBI GenBank Nucleotide #: NP_001005366.1
• UniProt Accession #: Q8NHM5
• Molecular Weight: Observed: 165 kDa; Predicted: 153 kDa
• NCBI Official Full Name: lysine-specific demethylase 2B isoform b
• NCBI Official Synonym Full Names: lysine demethylase 2B
• NCBI Official Symbol: KDM2B
• NCBI Official Synonym Symbols: CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
• NCBI Protein Information: lysine-specific demethylase 2B
• UniProt Protein Name: Lysine-specific demethylase 2B
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM2B
• UniProt Synonym Gene Names: CXXC2; FBL10; FBXL10; JHDM1B; PCCX2; Protein JEMMA
• UniProt Entry Name: KDM2B_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXL10: Histone demethylase that demethylates 'Lys-4' and 'Lys- 36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Belongs to the JHDM1 histone demethylase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.27; Oxidoreductase; Nucleolus; Demethylase; DNA-binding

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; nucleolus; PcG protein complex; nucleus

Molecular Function: rRNA binding; protein binding; histone demethylase activity; DNA binding; zinc ion binding; histone demethylase activity (H3-K36 specific)

Biological Process: midbrain-hindbrain boundary morphogenesis; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; fourth ventricle development; negative regulation of transcription from RNA polymerase II promoter; third ventricle development; initiation of neural tube closure; histone demethylation; midbrain development; forebrain development; spermatogenesis; embryonic camera-type eye morphogenesis; negative regulation of neuron apoptosis; lateral ventricle development; hindbrain development