SEPT4 sirna

SEPT4 siRNA (Human)

Cat. Num. AAA8235789

• Gene Names: SEPT4; H5; ARTS; MART; SEP4; CE5B3; PNUTL2; hucep-7; BRADEION; hCDCREL-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SEPT4; SEPT4 siRNA (Human); ARTS; PNUTL2; SEP4; Septin-4; Apoptosis-related protein in the TGF-beta signaling pathway; Bradeion beta; Brain protein H5; CE5B3 beta; Cell division control-related protein 2; hCDCREL-2; Cerebral protein 7; Peanut-like protein 2; SEPT4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SEPT4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 470

• Applicable Applications for SEPT4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SEPT4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SEPT4 sirna

siRNA to inhibit SEPT4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 311203834
• NCBI GeneID: 5414
• NCBI Accession #: NP_001185642.1
• NCBI GenBank Nucleotide #: NM_001198713.1
• UniProt Accession #: O43236; Q6IAP3; Q9H315; Q9UM58; B2RD42; B3KSX9; B4DXC6; B4DXV5
• Molecular Weight: 30,809 Da
• NCBI Official Full Name: septin-4 isoform 4
• NCBI Official Synonym Full Names: septin 4
• NCBI Official Symbol: SEPT4
• NCBI Official Synonym Symbols: H5; ARTS; MART; SEP4; CE5B3; PNUTL2; hucep-7; BRADEION; hCDCREL-2
• NCBI Protein Information: septin-4
• UniProt Protein Name: Septin-4
• UniProt Gene Name: SEPT4
• UniProt Synonym Gene Names: ARTS; PNUTL2; SEP4; ARTS; hCDCREL-2
• UniProt Entry Name: SEPT4_HUMAN

NCBI Description

This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010]

Uniprot Description

SEPT4: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli. Belongs to the septin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Hydrolase; Cell development/differentiation; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: cytoskeleton; mitochondrion; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural molecule activity

Biological Process: regulation of apoptosis; sperm mitochondrion organization and biogenesis; apoptosis; positive regulation of apoptosis; cell division; positive regulation of protein ubiquitination; metabolic process; brain development; cell cycle; sperm capacitation