SEC31A sirna

SEC31A siRNA (Human)

Cat. Num. AAA8239261

• Gene Names: SEC31A; ABP125; ABP130; HSPC275; HSPC334; SEC31L1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SEC31A; SEC31A siRNA (Human); KIAA0905; SEC31L1; Protein transport protein Sec31A; ABP125; ABP130; SEC31-like protein 1; SEC31-related protein A; Web1-like protein; SEC31A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SEC31A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1106

• Applicable Applications for SEC31A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SEC31A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SEC31A sirna

siRNA to inhibit SEC31A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116256336
• NCBI GeneID: 22872
• NCBI Accession #: NP_001070674.1
• NCBI GenBank Nucleotide #: NM_001077206.2
• UniProt Accession #: O94979; Q17RR5; Q5H9P6; Q5XG74; Q659G7; Q6ZU90; Q7LCX9; Q86TJ0; B4DIW6; B7ZKZ7; B7ZL00; H7C2W3
• Molecular Weight: 127,556 Da
• NCBI Official Full Name: protein transport protein Sec31A isoform 4
• NCBI Official Synonym Full Names: SEC31 homolog A, COPII coat complex component
• NCBI Official Symbol: SEC31A
• NCBI Official Synonym Symbols: ABP125; ABP130; HSPC275; HSPC334; SEC31L1
• NCBI Protein Information: protein transport protein Sec31A
• UniProt Protein Name: Protein transport protein Sec31A
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC31A
• UniProt Synonym Gene Names: KIAA0905; SEC31L1
• UniProt Entry Name: SC31A_HUMAN

NCBI Description

The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII , in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

SEC31A: Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. Interacts with PDCD6 in a calcium-dependent manner. Interacts with KLHL12. Abundantly and ubiquitously expressed. Belongs to the WD repeat SEC31 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Vesicle

Chromosomal Location of Human Ortholog: 4q21.22

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; COPII vesicle coat; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; ER to Golgi transport vesicle; cytosol

Molecular Function: protein binding; calcium-dependent protein binding

Biological Process: COPII coating of Golgi vesicle; protein transport; ER to Golgi vesicle-mediated transport; antigen processing and presentation of peptide antigen via MHC class I; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; response to calcium ion; post-translational protein modification