SEC24D sirna

SEC24D siRNA (Human)

Cat. Num. AAA8219503

• Gene Names: SEC24D; CLCRP2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SEC24D; SEC24D siRNA (Human); KIAA0755; Protein transport protein Sec24D; SEC24-related protein D; SEC24D sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SEC24D siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1032

• Applicable Applications for SEC24D sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SEC24D gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SEC24D sirna

siRNA to inhibit SEC24D expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 190343021
• NCBI GeneID: 9871
• NCBI Accession #: NP_055637.2
• NCBI GenBank Nucleotide #: NM_014822.2
• UniProt Accession #: O94855; Q8IYI7
• Molecular Weight: 113,081 Da
• NCBI Official Full Name: protein transport protein Sec24D
• NCBI Official Synonym Full Names: SEC24 homolog D, COPII coat complex component
• NCBI Official Symbol: SEC24D
• NCBI Official Synonym Symbols: CLCRP2
• NCBI Protein Information: protein transport protein Sec24D
• UniProt Protein Name: Protein transport protein Sec24D
• Protein Family: Protein transport protein
• UniProt Gene Name: SEC24D
• UniProt Synonym Gene Names: KIAA0755
• UniProt Entry Name: SC24D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. This gene product is implicated in the shaping of the vesicle, and also in cargo selection and concentration. [provided by RefSeq, Jul 2008]

Uniprot Description

SEC24D: Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. Interacts with TMED2 and TMED10. Ubiquitously expressed, with higher amounts in placenta, pancreas, heart and liver. Belongs to the SEC23/SEC24 family. SEC24 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; COPII vesicle coat; perinuclear region of cytoplasm; cytosol

Molecular Function: zinc ion binding

Biological Process: COPII coating of Golgi vesicle; intracellular protein transport; ER to Golgi vesicle-mediated transport; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; in utero embryonic development; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Cole-carpenter Syndrome 2