SCNN1G sirna

SCNN1G siRNA (Human)

Cat. Num. AAA8208934

• Gene Names: SCNN1G; PHA1; BESC3; ENaCg; SCNEG; ENaCgamma
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCNN1G; SCNN1G siRNA (Human); Amiloride-sensitive sodium channel subunit gamma; Epithelial Na(+) channel subunit gamma; ENaCG; Gamma-ENaC; Gamma-NaCH; Nonvoltage-gated sodium channel 1 subunit gamma; SCNEG; SCNN1G sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SCNN1G siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 649

• Applicable Applications for SCNN1G sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SCNN1G gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCNN1G sirna

siRNA to inhibit SCNN1G expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42476333
• NCBI GeneID: 6340
• NCBI Accession #: NP_001030.2
• NCBI GenBank Nucleotide #: NM_001039.3
• UniProt Accession #: P51170; P78437; Q6PCC2; Q93023; Q93024; Q93025; Q93026; Q93027; Q96TD2
• Molecular Weight: 74,270 Da
• NCBI Official Full Name: amiloride-sensitive sodium channel subunit gamma
• NCBI Official Synonym Full Names: sodium channel, non voltage gated 1 gamma subunit
• NCBI Official Symbol: SCNN1G
• NCBI Official Synonym Symbols: PHA1; BESC3; ENaCg; SCNEG; ENaCgamma
• NCBI Protein Information: amiloride-sensitive sodium channel subunit gamma
• UniProt Protein Name: Amiloride-sensitive sodium channel subunit gamma
• Protein Family: Amiloride-sensitive sodium channel
• UniProt Gene Name: SCNN1G
• UniProt Synonym Gene Names: ENaCG; Gamma-ENaC
• UniProt Entry Name: SCNNG_HUMAN

NCBI Description

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq, Apr 2009]

Uniprot Description

ENaC-gamma: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Defects in SCNN1G are a cause of Liddle syndrome (LIDDS). It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel. Defects in SCNN1G are the cause of bronchiectasis with or without elevated sweat chloride type 3 (BESC3). A debilitating respiratory disease characterized by chronic, abnormal dilatation of the bronchi and other cystic fibrosis-like symptoms in the absence of known causes of bronchiectasis (cystic fibrosis, autoimmune diseases, ciliary dyskinesia, common variable immunodeficiency, foreign body obstruction). Clinical features include sub-normal lung function, sinopulmonary infections, chronic productive cough, excessive sputum production, and elevated sweat chloride in some cases. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.

Protein type: Membrane protein, integral; Transporter, ion channel; Channel, sodium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: integral to plasma membrane; apical plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; protein binding; WW domain binding; sodium channel activity

Biological Process: sensory perception of taste; sodium ion transport; response to stimulus; sodium ion homeostasis; multicellular organismal water homeostasis; excretion; transmembrane transport

Disease: Bronchiectasis With Or Without Elevated Sweat Chloride 3; Pseudohypoaldosteronism, Type I, Autosomal Recessive; Liddle Syndrome