SCARB1 sirna

SCARB1 siRNA (Human)

Cat. Num. AAA8210103

• Gene Names: SCARB1; CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCARB1; SCARB1 siRNA (Human); CD36L1; CLA1; Scavenger receptor class B member 1; SRB1; CD36 and LIMPII analogous 1; CLA-1; CD36 antigen-like 1; Collagen type I receptor. thrombospondin receptor-like 1; SR-BI; CD antigen CD36; SCARB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SCARB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 506

• Applicable Applications for SCARB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SCARB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCARB1 sirna

siRNA to inhibit SCARB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 132566680
• NCBI GeneID: 949
• NCBI Accession #: NP_001076428.1
• NCBI GenBank Nucleotide #: NM_001082959.1
• UniProt Accession #: Q8WTV0; Q14016; Q52LZ5; Q6KFX4; F8W8N0
• Molecular Weight: 56,170 Da
• NCBI Official Full Name: scavenger receptor class B member 1 isoform 2
• NCBI Official Synonym Full Names: scavenger receptor class B, member 1
• NCBI Official Symbol: SCARB1
• NCBI Official Synonym Symbols: CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
• NCBI Protein Information: scavenger receptor class B member 1
• UniProt Protein Name: Scavenger receptor class B member 1
• Protein Family: Scavenger receptor
• UniProt Gene Name: SCARB1
• UniProt Synonym Gene Names: CD36L1; CLA1; SRB1; CLA-1
• UniProt Entry Name: SCRB1_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

SCARB1: Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. Belongs to the CD36 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell surface; integral to plasma membrane; microvillus membrane; lysosomal membrane; plasma membrane; caveola

Molecular Function: apolipoprotein A-I binding; protein binding; protein homodimerization activity; transporter activity; phosphatidylserine binding; lipopolysaccharide binding; low-density lipoprotein binding; lipopolysaccharide receptor activity; apolipoprotein binding; high-density lipoprotein binding; phosphatidylinositol binding

Biological Process: receptor-mediated endocytosis; blood vessel endothelial cell migration; wound healing; viral reproduction; positive regulation of nitric-oxide synthase activity; cholesterol efflux; lipoprotein metabolic process; androgen biosynthetic process; regulation of phagocytosis; recognition of apoptotic cell; lipopolysaccharide transport; cholesterol homeostasis; reverse cholesterol transport; endothelial cell proliferation; detection of lipopolysaccharide; lipopolysaccharide-mediated signaling pathway; adhesion to host; phospholipid transport; cholesterol catabolic process; cell adhesion

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 6