SCAI sirna

SCAI siRNA (Human)

Cat. Num. AAA8202527

• Gene Names: SCAI; NET40; C9orf126
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCAI; SCAI siRNA (Human); C9orf126; Protein SCAI; Suppressor of cancer cell invasion protein; SCAI sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SCAI siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 606

• Applicable Applications for SCAI sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SCAI gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCAI sirna

siRNA to inhibit SCAI expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 221554543
• NCBI GeneID: 286205
• NCBI Accession #: NP_001138349.1
• NCBI GenBank Nucleotide #: NM_001144877.2
• UniProt Accession #: Q8N9R8; Q3SXZ1; Q3SXZ2; Q5T163; Q8N1I4
• Molecular Weight: 72,934 Da
• NCBI Official Full Name: protein SCAI isoform 2
• NCBI Official Synonym Full Names: suppressor of cancer cell invasion
• NCBI Official Symbol: SCAI
• NCBI Official Synonym Symbols: NET40; C9orf126
• NCBI Protein Information: protein SCAI
• UniProt Protein Name: Protein SCAI
• Protein Family: Protein
• UniProt Gene Name: SCAI
• UniProt Synonym Gene Names: C9orf126
• UniProt Entry Name: SCAI_HUMAN

NCBI Description

This gene encodes a regulator of cell migration. The encoded protein appears to function in the RhoA (ras homolog gene family, member A)-Dia1 (diaphanous homolog 1) signal transduction pathway. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Uniprot Description

SCAI: Tumor suppressor which functions to suppress MKL1- induced SRF transcriptional activity. May function in the RHOA- DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1. Belongs to the SCAI family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nuclear membrane; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: regulation of transcription, DNA-dependent; negative regulation of Rho protein signal transduction; transcription, DNA-dependent; negative regulation of cell migration