RRAS2 sirna

RRAS2 siRNA (Human)

Cat. Num. AAA8237181

• Gene Names: RRAS2; TC21
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RRAS2; RRAS2 siRNA (Human); TC21; Ras-related protein R-Ras2; Ras-like protein TC21; Teratocarcinoma oncogene; RRAS2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RRAS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 210

• Applicable Applications for RRAS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RRAS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RRAS2 sirna

siRNA to inhibit RRAS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156713451
• NCBI GeneID: 22800
• NCBI Accession #: NP_001096139.1
• NCBI GenBank Nucleotide #: NM_001102669.2
• UniProt Accession #: P62070; P17082; B2R9Z3; B7Z5Z2; B7Z6C4; B7Z7H6
• Molecular Weight: 24,196 Da
• NCBI Official Full Name: ras-related protein R-Ras2 isoform b
• NCBI Official Synonym Full Names: related RAS viral (r-ras) oncogene homolog 2
• NCBI Official Symbol: RRAS2
• NCBI Official Synonym Symbols: TC21
• NCBI Protein Information: ras-related protein R-Ras2
• UniProt Protein Name: Ras-related protein R-Ras2
• Protein Family: Ras-related protein
• UniProt Gene Name: RRAS2
• UniProt Synonym Gene Names: TC21
• UniProt Entry Name: RRAS2_HUMAN

NCBI Description

This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

RRAS2: It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion. Defects in RRAS2 are a cause of susceptibility to ovarian cancer (OC). The term ovarian cancer defines common malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, monomeric, Ras; Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: focal adhesion; membrane; endoplasmic reticulum; plasma membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: osteoblast differentiation; metabolic process; Ras protein signal transduction; positive regulation of cell migration