RPS6KA2 sirna

RPS6KA2 siRNA (Human)

Cat. Num. AAA8220924

• Gene Names: RPS6KA2; RSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPS6KA2; RPS6KA2 siRNA (Human); MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; p90RSK2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; Ribosomal S6 kinase 3; RSK-3; pp90RSK3; RPS6KA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RPS6KA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 741

• Applicable Applications for RPS6KA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RPS6KA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPS6KA2 sirna

siRNA to inhibit RPS6KA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55743136
• NCBI GeneID: 6196
• NCBI Accession #: NP_001006933.1
• NCBI GenBank Nucleotide #: NM_001006932.1
• UniProt Accession #: Q15349; Q15419; Q59GJ3; Q5TI68; Q96J38; Q9UJN5; B3KTK9
• Molecular Weight: 84,014 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-2 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 2
• NCBI Official Symbol: RPS6KA2
• NCBI Official Synonym Symbols: RSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
• NCBI Protein Information: ribosomal protein S6 kinase alpha-2
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-2
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA2
• UniProt Synonym Gene Names: MAPKAPK1C; RSK3; S6K-alpha-2; p90-RSK 2; p90RSK2; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; RSK-3
• UniProt Entry Name: KS6A2_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

RSK3: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6.

Protein type: Tumor suppressor; Protein kinase, AGC; Translation; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; protein serine/threonine/tyrosine kinase activity; ribosomal protein S6 kinase activity; ATP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; heart development; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; signal transduction; oocyte maturation; brain renin-angiotensin system; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; negative regulation of cell cycle; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; synaptic transmission; negative regulation of cell proliferation; peptidyl-serine phosphorylation; heart contraction; negative regulation of meiosis; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway