LECT1 Recombinant Protein | CNMD recombinant protein

LECT1 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121389

• Gene Names: LECT1; CHM1; CHM-I; BRICD3; MYETS1
• Purity: >(81.7+13.6)% as determined by SDS-PAGE
• Synonyms: LECT1; LECT1 Protein; Human; Recombinant (hFc Tag); Human LECT1 Protein (Fc Tag); BRICD3 Protein; CHM-I Protein; CHM1 Protein; MYETS1 Protein; leukocyte cell derived chemotaxin 1; CNMD recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >(81.7+13.6)% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 5% Trehalose, 5% mannitol, 0.01% Tween-80
• Sequence: Glu215-Val333

• Source: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu
• Tag: Fc
• Protein Construction: A DNA sequence encoding the human LECT1 (NP_001011705.1)(Glu215-Val333) was expressed with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL.; Concentration is mesured by UV-Vis
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C upon receipt. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.,br>Stability: Samples are stabel for up to 12 months from date of receipt at -70°C.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 59806343
• NCBI GeneID: 11061
• NCBI Accession #: NP_001011705.1
• NCBI GenBank Nucleotide #: NM_001011705.1
• UniProt Accession #: O75829; Q5TAM4; Q8TAY6; Q9UM18
• Molecular Weight: The recombinant human LECT1 consists 379 amino acids and predicts a molecular mass of 42.1 kDa.
• NCBI Official Full Name: leukocyte cell-derived chemotaxin 1 isoform 2
• NCBI Official Synonym Full Names: leukocyte cell derived chemotaxin 1
• NCBI Official Symbol: LECT1
• NCBI Official Synonym Symbols: CHM1; CHM-I; BRICD3; MYETS1
• NCBI Protein Information: leukocyte cell-derived chemotaxin 1; BRICHOS domain containing 3; chondromodulin I; chondromodulin-1; chondromodulin-I; multiple myeloma tumor suppressor 1
• UniProt Protein Name: Leukocyte cell-derived chemotaxin 1
• UniProt Gene Name: LECT1
• UniProt Synonym Gene Names: CHMI; CH-SP; ChM-I
• UniProt Entry Name: LECT1_HUMAN

NCBI Description

This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

LECT1: Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization. Belongs to the chondromodulin-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: proteinaceous extracellular matrix; integral to membrane; endomembrane system

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; negative regulation of vascular endothelial growth factor receptor signaling pathway; cartilage development; negative regulation of endothelial cell proliferation; endothelial cell morphogenesis; skeletal development