RIN1 sirna

RIN1 siRNA (Human)

Cat. Num. AAA8222526

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RIN1; RIN1 siRNA (Human); Ras and Rab interactor 1; Ras inhibitor JC99; Ras interaction/interference protein 1; RIN1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RIN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 783

• Applicable Applications for RIN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RIN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RIN1 sirna

siRNA to inhibit RIN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 68989256
• NCBI GeneID: 9610
• NCBI Accession #: NP_004283.2
• NCBI GenBank Nucleotide #: NM_004292.2
• UniProt Accession #: Q13671; O15010; Q00427; Q96CC8
• Molecular Weight: 77,500 Da
• NCBI Official Full Name: ras and Rab interactor 1
• NCBI Official Synonym Full Names: Ras and Rab interactor 1
• NCBI Official Symbol: RIN1
• NCBI Protein Information: ras and Rab interactor 1
• UniProt Protein Name: Ras and Rab interactor 1
• Protein Family: RuvB-like protein
• UniProt Gene Name: RIN1
• UniProt Entry Name: RIN1_HUMAN

Uniprot Description

Rin1: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis. Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351. Expressed in all tissues examined with high levels in brain, placenta and pancreas. Belongs to the RIN (Ras interaction/interference) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rab; GEFs

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytoskeleton; cell soma; cytoplasm; dendrite; plasma membrane

Molecular Function: protein binding; Ras GTPase binding; GTPase activator activity

Biological Process: negative regulation of synaptic plasticity; endocytosis; signal transduction; associative learning; memory; positive regulation of GTPase activity