RCOR1 sirna

RCOR1 siRNA (Human)

Cat. Num. AAA8228181

• Gene Names: RCOR1; RCOR; COREST
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RCOR1; RCOR1 siRNA (Human); KIAA0071; RCOR; REST corepressor 1; Protein CoREST; RCOR1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RCOR1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 485

• Applicable Applications for RCOR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RCOR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RCOR1 sirna

siRNA to inhibit RCOR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 344925845
• NCBI GeneID: 23186
• NCBI Accession #: NP_055971.2
• NCBI GenBank Nucleotide #: NM_015156.3
• UniProt Accession #: Q9UKL0; Q15044; Q6P2I9; Q86VG5
• Molecular Weight: 53,028 Da
• NCBI Official Full Name: REST corepressor 1
• NCBI Official Synonym Full Names: REST corepressor 1
• NCBI Official Symbol: RCOR1
• NCBI Official Synonym Symbols: RCOR; COREST
• NCBI Protein Information: REST corepressor 1
• UniProt Protein Name: REST corepressor 1
• Protein Family: REST corepressor
• UniProt Gene Name: RCOR1
• UniProt Synonym Gene Names: KIAA0071; RCOR
• UniProt Entry Name: RCOR1_HUMAN

NCBI Description

This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor). [provided by RefSeq, Aug 2011]

Uniprot Description

RCOR1: Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Belongs to the CoREST family.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 14q32.31

Cellular Component: nucleoplasm; transcriptional repressor complex; nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; erythrocyte differentiation; positive regulation of megakaryocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; blood coagulation; negative regulation of transcription, DNA-dependent