PTPMT1 sirna

PTPMT1 siRNA (Human)

Cat. Num. AAA829341

• Gene Names: PTPMT1; MOSP; PLIP; DUSP23; PNAS-129
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPMT1; PTPMT1 siRNA (Human); MOSP; PLIP; Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1; PTEN-like phosphatase; Phosphoinositide lipid phosphatase; Protein-tyrosine phosphatase mitochondrial 1; PTPMT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PTPMT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 151

• Applicable Applications for PTPMT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PTPMT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPMT1 sirna

siRNA to inhibit PTPMT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 221218988
• NCBI GeneID: 114971
• NCBI Accession #: NP_001137456.1
• NCBI GenBank Nucleotide #: NM_001143984.1
• UniProt Accession #: Q8WUK0; Q7Z557; Q96CR2; Q9BXV8; E9PAT8
• Molecular Weight: 16,459 Da
• NCBI Official Full Name: phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, mitochondrial 1
• NCBI Official Symbol: PTPMT1
• NCBI Official Synonym Symbols: MOSP; PLIP; DUSP23; PNAS-129
• NCBI Protein Information: phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1
• UniProt Protein Name: Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1
• Protein Family: Phosphatidylglycerophosphatase and protein-tyrosine phosphatase
• UniProt Gene Name: PTPMT1
• UniProt Synonym Gene Names: MOSP; PLIP
• UniProt Entry Name: PTPM1_HUMAN

Uniprot Description

PTPMT1: Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG). PGP is an essential intermediate in the biosynthetic pathway of cardiolipin, a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle. Has also been shown to display phosphatase activity toward phosphoprotein substrates, specifically mediates dephosphorylation of mitochondrial proteins, thereby playing an essential role in ATP production. Has probably a preference for proteins phosphorylated on Ser and/or Thr residues compared to proteins phosphorylated on Tyr residues. Probably involved in regulation of insulin secretion in pancreatic beta cells. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Mitochondrial; EC 3.1.3.16; Phosphatase, lipid; Protein phosphatase, dual-specificity; EC 3.1.3.27

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: mitochondrion; integral to mitochondrial inner membrane; nucleus

Molecular Function: phosphoinositide 5-phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; phosphatidylglycerophosphatase activity; protein tyrosine phosphatase activity

Biological Process: cardiolipin biosynthetic process; phosphatidylglycerol biosynthetic process; inositol phosphate dephosphorylation; phospholipid metabolic process; glycerophospholipid biosynthetic process; protein amino acid dephosphorylation