PTPMT1 active protein

PTPMT1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121237

• Gene Names: PTPMT1; MOSP; PLIP; DUSP23; PNAS-129
• Purity: >94% as determined by SDS-PAGE
• Synonyms: PTPMT1; PTPMT1 Protein; Human; Recombinant (His Tag); Human PTPMT1 Protein (His Tag); DUSP23 Protein; FLJ46081 Protein; MOSP Protein; PLIP Protein; PNAS-129 Protein; protein tyrosine phosphatase; mitochondrial 1; PTPMT1 active protein

• Host: E Coli
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% glycerol, 1mM DTT, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys28-Thr201

• Species: Human
• Activity: Measured by its ability to cleave pNPP. The specific activity is >200pmoles/min/ug.
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PTPMT1 isoform 1 (Q8WUK0-1)(Lys 28-Thr 201) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PTPMT1 active protein

Background: PTPMT1 (PTP localized to the Mitochondrion 1) is a member of the protein tyrosine phosphatase superfamily that is localized exclusively to the mitochondrion. It has been recently reported that PTPMT1 dephosphorylates phosphatidylglycerol phosphate, an essential intermediate of cardiolipin biosynthesis. PTPMT1 deficiency in mouse embryonic fibroblasts compromises mitochondrial respiration and results in abnormal mitochondrial morphology. Lipid analysis of PTPMT1-deficient fibroblasts reveals an accumulation of PGP along with a concomitant decrease in phosphatidylglycerol. Modulation of mitochondrial ATP synthesis by PTPMT1 suggests a novel approach for the treatment of pancreatic cancers, which represent some of the deadliest forms of human tumors. The gluttony of cancer cells for energy is well established, and with the development of a modulator of expression, one may hope that we could also achieve the synthetic induction of PTPMT1 expression. It would then be expected that this effect would attenuate, if not abolish, the growth of pancreas-derived tumor cells and support the establishment of a novel regimen for pancreatic cancers.

References

Zhang J, et al. (2011) Mitochondrial phosphatase PTPMT1 is essential for cardiolipin biosynthesis. Cell Metab. 13 (6): 690-700.Boisclair Y, et al. (2005) Firing up mitochondrial activities with PTPMT1. Mol Cell. 19 (3): 291-2.Xiao J, et al. (2011) Structural and functional analysis of PTPMT1, a phosphatase required for cardiolipin synthesis. Proc Natl Acad Sci. 108 (29): 11860-5.

NCBI and Uniprot Product Information

• NCBI GI #: 221218988
• NCBI GeneID: 114971
• NCBI Accession #: NP_001137456.1
• NCBI GenBank Nucleotide #: NM_001143984.1
• UniProt Accession #: Q8WUK0; Q7Z557; Q96CR2; Q9BXV8; E9PAT8
• Molecular Weight: 16,459 Da
• NCBI Official Full Name: phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, mitochondrial 1
• NCBI Official Symbol: PTPMT1
• NCBI Official Synonym Symbols: MOSP; PLIP; DUSP23; PNAS-129
• NCBI Protein Information: phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1; NB4 apoptosis/differentiation related protein; PTEN-like phosphatase; phosphoinositide lipid phosphatase; protein-tyrosine phosphatase mitochondrial 1
• UniProt Protein Name: Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1
• Protein Family: Phosphatidylglycerophosphatase and protein-tyrosine phosphatase
• UniProt Gene Name: PTPMT1
• UniProt Synonym Gene Names: MOSP; PLIP
• UniProt Entry Name: PTPM1_HUMAN

Uniprot Description

PTPMT1: Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG). PGP is an essential intermediate in the biosynthetic pathway of cardiolipin, a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle. Has also been shown to display phosphatase activity toward phosphoprotein substrates, specifically mediates dephosphorylation of mitochondrial proteins, thereby playing an essential role in ATP production. Has probably a preference for proteins phosphorylated on Ser and/or Thr residues compared to proteins phosphorylated on Tyr residues. Probably involved in regulation of insulin secretion in pancreatic beta cells. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.16; Phosphatase, lipid; EC 3.1.3.27; EC 3.1.3.48; Mitochondrial

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: mitochondrion; integral to mitochondrial inner membrane; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; phosphoinositide 5-phosphatase activity; protein tyrosine phosphatase activity; phosphatidylglycerophosphatase activity

Biological Process: cardiolipin biosynthetic process; phosphatidylglycerol biosynthetic process; inositol phosphate dephosphorylation; phospholipid metabolic process; glycerophospholipid biosynthetic process; protein amino acid dephosphorylation