PRKAR2A sirna

PRKAR2A siRNA (Human)

Cat. Num. AAA8212808

• Gene Names: PRKAR2A; PKR2; PRKAR2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRKAR2A; PRKAR2A siRNA (Human); PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRKAR2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 404

• Applicable Applications for PRKAR2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRKAR2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRKAR2A sirna

siRNA to inhibit PRKAR2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4758958
• NCBI GeneID: 5576
• NCBI Accession #: NP_004148.1
• NCBI GenBank Nucleotide #: NM_004157.2
• UniProt Accession #: P13861; Q16823; Q9BUB1
• Molecular Weight: 43,067 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type II, alpha
• NCBI Official Symbol: PRKAR2A
• NCBI Official Synonym Symbols: PKR2; PRKAR2
• NCBI Protein Information: cAMP-dependent protein kinase type II-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR2A
• UniProt Synonym Gene Names: PKR2; PRKAR2
• UniProt Entry Name: KAP2_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Uniprot Description

PKAR2A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 3p21.3-p21.2

Cellular Component: centrosome; focal adhesion; membrane; cytoplasm; plasma membrane; cytosol; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase inhibitor activity; ubiquitin protein ligase binding; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; energy reserve metabolic process; renal water homeostasis; innate immune response; blood coagulation; signal transduction; transmembrane transport; regulation of insulin secretion