PPAP2B sirna

PPAP2B siRNA (Human)

Cat. Num. AAA8234285

• Gene Names: PPAP2B; LPP3; VCIP; Dri42; PAP2B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPAP2B; PPAP2B siRNA (Human); LPP3; Lipid phosphate phosphohydrolase 3; PAP2-beta; Phosphatidate phosphohydrolase type 2b; Phosphatidic acid phosphatase 2b; PAP-2b; PAP2b; Vascular endothelial growth factor and type I collagen-inducible protein; VCIP; PPAP2B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PPAP2B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 311

• Applicable Applications for PPAP2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PPAP2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPAP2B sirna

siRNA to inhibit PPAP2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 29171740
• NCBI GeneID: 8613
• NCBI Accession #: NP_003704.3
• NCBI GenBank Nucleotide #: NM_003713.4
• UniProt Accession #: O14495; Q5U0F7; Q96GW0; Q99782; B2R651; D3DQ52
• Molecular Weight: 35,116 Da
• NCBI Official Full Name: lipid phosphate phosphohydrolase 3
• NCBI Official Synonym Full Names: phosphatidic acid phosphatase type 2B
• NCBI Official Symbol: PPAP2B
• NCBI Official Synonym Symbols: LPP3; VCIP; Dri42; PAP2B
• NCBI Protein Information: lipid phosphate phosphohydrolase 3
• UniProt Protein Name: Lipid phosphate phosphohydrolase 3
• UniProt Gene Name: PPAP2B
• UniProt Synonym Gene Names: LPP3; PAP-2b; PAP2b; VCIP
• UniProt Entry Name: LPP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. [provided by RefSeq, Mar 2010]

Uniprot Description

PPAP2B: Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1- phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions. Belongs to the PA-phosphatase related phosphoesterase family.

Protein type: Membrane protein, multi-pass; Lipid Metabolism - ether lipid; Lipid Metabolism - sphingolipid; Lipid Metabolism - glycerolipid; Phosphatase, lipid; Membrane protein, integral; Lipid Metabolism - glycerophospholipid; EC 3.1.3.4; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p32.2

Cellular Component: Golgi apparatus; adherens junction; membrane; integral to membrane; plasma membrane

Molecular Function: integrin binding; protein binding; phosphatidate phosphatase activity; lipid phosphatase activity; phosphoprotein phosphatase activity; sphingosine-1-phosphate phosphatase activity

Biological Process: blood vessel development; sphingolipid metabolic process; protein stabilization; sphingolipid biosynthetic process; regulation of Wnt receptor signaling pathway; gastrulation with mouth forming second; Bergmann glial cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; dephosphorylation; negative regulation of protein amino acid phosphorylation; phospholipid metabolic process; germ cell migration; homotypic cell-cell adhesion; positive regulation of transcription factor activity; lipid metabolic process