POU3F2 sirna

POU3F2 siRNA (Human)

Cat. Num. AAA8218836

• Gene Names: POU3F2; BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POU3F2; POU3F2 siRNA (Human); BRN2; OCT7; OTF7; POU domain. class 3. transcription factor 2; Brain-specific homeobox/POU domain protein 2; Brain-2; Brn-2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Octamer-binding protein 7; Oct-7; Octamer-binding transcription factor 7; OTF-7; POU3F2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POU3F2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 443

• Applicable Applications for POU3F2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POU3F2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POU3F2 sirna

siRNA to inhibit POU3F2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51702521
• NCBI GeneID: 5454
• NCBI Accession #: NP_005595.2
• NCBI GenBank Nucleotide #: NM_005604.3
• UniProt Accession #: P20265; Q14960; Q86V54; Q9UJL0
• Molecular Weight: 26,841 Da
• NCBI Official Full Name: POU domain, class 3, transcription factor 2
• NCBI Official Synonym Full Names: POU class 3 homeobox 2
• NCBI Official Symbol: POU3F2
• NCBI Official Synonym Symbols: BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3
• NCBI Protein Information: POU domain, class 3, transcription factor 2
• UniProt Protein Name: POU domain, class 3, transcription factor 2
• Protein Family: POU domain, class 3, transcription factor
• UniProt Gene Name: POU3F2
• UniProt Synonym Gene Names: BRN2; OCT7; OTF7; Brain-2; Brn-2; Oct-7; OTF-7
• UniProt Entry Name: PO3F2_HUMAN

NCBI Description

This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012]

Uniprot Description

Oct7: Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. Interacts with PQBP1. Expressed specifically in the neuroectodermal cell lineage. Belongs to the POU transcription factor family. Class- 3 subfamily. 3 isoforms of the human protein are produced by alternative initiation.

Protein type: Motility/polarity/chemotaxis; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: transcription factor complex; nucleus

Molecular Function: identical protein binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: epidermis development; transcription, DNA-dependent; hypothalamus cell differentiation; positive regulation of multicellular organism growth; regulation of axonogenesis; astrocyte development; myelination in the peripheral nervous system; neuron differentiation; neurohypophysis development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex radially oriented cell migration; forebrain ventricular zone progenitor cell division