Rabbit anti-Human NR1I3 / CAR Polyclonal Antibody | anti-NR1I3 antibody

Rabbit Polyclonal to Human NR1I3 / CAR

Cat. Num. AAA242139

• Gene Names: NR1I3; CAR; CAR1; MB67
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NR1I3 / CAR; Polyclonal Antibody; Rabbit Polyclonal to Human NR1I3 / CAR; Anti-NR1I3 / CAR Antibody (N-Terminus) IHC-plus; NR1I3; Car-beta; CAR1; Constitutive active receptor; Constitutive active response; Orphan nuclear receptor MB67; MB67; CAR; Human NR1I3; anti-NR1I3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: synthetic peptide corresponding to N-terminal residues of human CNG3(NR1I3 (Orphan nuclear receptor NR1I3)
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.01% sodium azide, 50% glycerol.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 348

• Applicable Applications for anti-NR1I3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 - 20 ug/ml), WB (1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human NR1I3.
• Immunogen Type: Synthetic peptide
• Immunogen: NR1I3 / CAR antibody was raised against synthetic peptide from human NR1I3.
• Antigen Modification: N-Terminus
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NR1I3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Related Product Information for anti-NR1I3 antibody

Constitutive androstane receptor (CAR), a NR1 Thyroid Hormone-Like Receptor, has been shown to affect steroid homeostasis by mediating the expression of CYP genes to xenochemicals, steroids, and lipids. CAR activity is suppressed upon ligand binding; however, the suppression is reversed by phenobarbital. CAR binds to the DR5 hormone response element as a heterodimer (with retinoid X receptor) and can activate genes in the absence of retinoids, rexinoids, or exogenous ligands.

Product Categories/Family for anti-NR1I3 antibody

Family: NHR; Subfamily: NR1 Thyroid hormone-like

NCBI and Uniprot Product Information

• NCBI GI #: 4826661
• NCBI GeneID: 9970
• NCBI Accession #: NP_005113.1
• NCBI GenBank Nucleotide #: NM_005122.4
• UniProt Accession #: Q14994; Q0VAC9; Q4U0F0; E9PB75; E9PC13; E9PDU3; E9PGH6; E9PH10; E9PHC8; E9PHN4; F1D8Q0; F1D8Q1
• Molecular Weight: 30,262 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group I member 3 isoform 3
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group I, member 3
• NCBI Official Symbol: NR1I3
• NCBI Official Synonym Symbols: CAR; CAR1; MB67
• NCBI Protein Information: nuclear receptor subfamily 1 group I member 3; constitutive active receptor; constitutive active response; orphan nuclear receptor MB67; orphan nuclear hormone receptor; constitutive androstane receptor; constitutive activator of retinoid response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5
• UniProt Protein Name: Nuclear receptor subfamily 1 group I member 3
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1I3
• UniProt Synonym Gene Names: CAR; Constitutive active response; CAR
• UniProt Entry Name: NR1I3_HUMAN

NCBI Description

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; cytosol; nucleus

Molecular Function: ligand-dependent nuclear receptor activity; androgen receptor activity; DNA binding; zinc ion binding; transcription coactivator activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; androgen receptor signaling pathway; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent