PLP1 sirna

PLP1 siRNA (Human)

Cat. Num. AAA829081

• Gene Names: PLP1; PLP; PMD; HLD1; MMPL; SPG2; GPM6C; PLP/DM20
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLP1; PLP1 siRNA (Human); PLP; Myelin proteolipid protein; Lipophilin; PLP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 277

• Applicable Applications for PLP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLP1 sirna

siRNA to inhibit PLP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41349499
• NCBI GeneID: 5354
• NCBI Accession #: NP_000524.3
• NCBI GenBank Nucleotide #: NM_000533.4
• UniProt Accession #: P60201; P04400; P06905; Q502Y1; Q6FHZ6
• Molecular Weight: 26,274 Da
• NCBI Official Full Name: myelin proteolipid protein isoform 1
• NCBI Official Synonym Full Names: proteolipid protein 1
• NCBI Official Symbol: PLP1
• NCBI Official Synonym Symbols: PLP; PMD; HLD1; MMPL; SPG2; GPM6C; PLP/DM20
• NCBI Protein Information: myelin proteolipid protein
• UniProt Protein Name: Myelin proteolipid protein
• Protein Family: Patatin-like protein
• UniProt Gene Name: PLP1
• UniProt Synonym Gene Names: PLP; PLP
• UniProt Entry Name: MYPR_HUMAN

NCBI Description

This gene encodes a transmembrane proteolipid protein that is the predominant component of myelin. The encoded protein may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively splicing results in multiple transcript variants, including the DM20 splice variant. [provided by RefSeq, Feb 2015]

Uniprot Description

PLP1: This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1); also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay. Defects in PLP1 are the cause of spastic paraplegia X- linked type 2 (SPG2). SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. Belongs to the myelin proteolipid protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: plasma membrane; integral to membrane; myelin sheath

Molecular Function: structural constituent of myelin sheath; structural molecule activity

Biological Process: integrin-mediated signaling pathway; synaptic transmission; substantia nigra development; myelination in the central nervous system; cell maturation; long-chain fatty acid biosynthetic process; axon ensheathment; inflammatory response; astrocyte development

Disease: Spastic Paraplegia 2, X-linked; Pelizaeus-merzbacher Disease