PP1-alpha Antibody | anti-PP1-alpha antibody

PP1-alpha (Ab-320) Antibody

Cat. Num. AAA8509583

• Gene Names: PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PP1-alpha; PP1-alpha (Ab-320) Antibody; EC 3.1.3.16; PP-1A; PP1A; PPP1A; Serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; Serine/threonine protein phosphatase PP1-alpha 1; catalytic subunit; anti-PP1-alpha antibody

• Reactivity: Human, Mouse, Rat
• Specificity: PP1-alpha (Ab-320) antibody detects endogenous levels of total PP1-alpha protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 341

• Applicable Applications for anti-PP1-alpha antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human PP1-alpha around the phosphorylation site of threonine 320 (P-I-TP-P-P).
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using PP1-alpha (Ab-320) antibody.)

References

Song Q., Gene 129:291-295(1993). Ceulemans H., J. Biol. Chem. 277:47331-47337(2002). Cohen P.T., J. Cell Sci. 115:241-256(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 56790945
• NCBI GeneID: 5499
• NCBI Accession #: NP_001008709.1
• NCBI GenBank Nucleotide #: NM_001008709.1
• UniProt Accession #: P62136; P08129; P20653; P22802; Q07161; A6NNR3; B2R908
• Molecular Weight: 32,595 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 3
• NCBI Official Synonym Full Names: protein phosphatase 1 catalytic subunit alpha
• NCBI Official Symbol: PPP1CA
• NCBI Official Synonym Symbols: PP1A; PP-1A; PPP1A; PP1alpha
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Gene Name: PPP1CA
• UniProt Synonym Gene Names: PPP1A; PP-1A

NCBI Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).