Western Blot (WB)
(Fig: 1 Western blot analysis of NALP2 using NALP2 antibody at 1: 2000. HEK293T: mock transfected cells, HEK293T-NALP2: NALP2 transiently transfected cells; THP-1: untreated cells; THP1-LPS: cells treated with 1 ug/ml LPS for 4h. MCF-7: untreated cells.)
Rabbit anti-Human NLRP2/NALP2 Polyclonal Antibody | anti-NLRP2 antibody
Polyclonal Antibody to NLRP2/NALP2 (PAN1)
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig: 1 Western blot analysis of NALP2 using NALP2 antibody at 1: 2000. HEK293T: mock transfected cells, HEK293T-NALP2: NALP2 transiently transfected cells; THP-1: untreated cells; THP1-LPS: cells treated with 1 ug/ml LPS for 4h. MCF-7: untreated cells.)
Western Blot (WB)
(Fig: 1 Western blot analysis of NALP2 using NALP2 antibody at 1: 2000. HEK293T: mock transfected cells, HEK293T-NALP2: NALP2 transiently transfected cells; THP-1: untreated cells; THP1-LPS: cells treated with 1 ug/ml LPS for 4h. MCF-7: untreated cells.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]
Uniprot Description
NLRP2: Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nuclear chromosome, telomeric region
Molecular Function: protein binding; Pyrin domain binding
Biological Process: positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion
Research Articles on NLRP2
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Product Notes
The NLRP2 nlrp2 (Catalog #AAA668735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to NLRP2/NALP2 (PAN1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NLRP2/NALP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200. Researchers should empirically determine the suitability of the NLRP2 nlrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLRP2/NALP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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