PLEKHG4 sirna

PLEKHG4 siRNA (Human)

Cat. Num. AAA8204405

• Gene Names: PLEKHG4; SCA4; PRTPHN1; ARHGEF44
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLEKHG4; PLEKHG4 siRNA (Human); PRTPHN1; Puratrophin-1; Pleckstrin homology domain-containing family G member 4; PH domain-containing family G member 4; Purkinje cell atrophy-associated protein 1; PLEKHG4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLEKHG4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1191

• Applicable Applications for PLEKHG4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLEKHG4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLEKHG4 sirna

siRNA to inhibit PLEKHG4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 193211594
• NCBI GeneID: 25894
• NCBI Accession #: NP_001123199.1
• NCBI GenBank Nucleotide #: NM_001129727.2
• UniProt Accession #: Q58EX7; Q4G0J8; Q4H485; Q56A69; Q9H7K4; Q9UFW0
• Molecular Weight: 20,247 Da
• NCBI Official Full Name: puratrophin-1 isoform 1
• NCBI Official Synonym Full Names: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
• NCBI Official Symbol: PLEKHG4
• NCBI Official Synonym Symbols: SCA4; PRTPHN1; ARHGEF44
• NCBI Protein Information: puratrophin-1
• UniProt Protein Name: Puratrophin-1
• Protein Family: Puratrophin
• UniProt Gene Name: PLEKHG4
• UniProt Synonym Gene Names: PRTPHN1; PH domain-containing family G member 4
• UniProt Entry Name: PKHG4_HUMAN

NCBI Description

The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

PLEKHG4: a guanine nucleotide exchange factor (GEF) that facilitates activation of the small GTPases Rac1, Cdc42, and RhoA. A member of the Dbl family GEFs that regulates GTPase signaling critical for proper cerebellar function. Its expression is developmentally regulated in the murine brain, with pronounced expression in the newborn midbrain and brainstem that wanes with age and maximal expression in adult cerebellar Purkinje neurons. Widely expressed at low levels. More strongly expressed in testis and pancreas. Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. May play a role in cytoskeleton dynamics at the Golgi. Overexpression in NIH3T3 cells induces rearrangements of the actin cytoskeleton including enhanced formation of lamellopodia and fillopodia. Three isoforms of the human protein are produced by alternative splicing. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons.

Protein type: GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 16q22.1

Molecular Function: Rho guanyl-nucleotide exchange factor activity

Disease: Spinocerebellar Ataxia 31; Spinocerebellar Ataxia 4