Western Blot (WB)
(Western blot analysis of PLEKHG4 expression in SHSY5Y (A), HEK293T (B), K562 (C) whole cell lysates.)
Rabbit PLEKHG4 Polyclonal Antibody | anti-PLEKHG4 antibody
Anti-PLEKHG4 Antibody
Western Blot (WB)
(Western blot analysis of PLEKHG4 expression in SHSY5Y (A), HEK293T (B), K562 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PLEKHG4 expression in SHSY5Y (A), HEK293T (B), K562 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PLEKHG4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PLEKHG4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Uniprot Description
PLEKHG4: a guanine nucleotide exchange factor (GEF) that facilitates activation of the small GTPases Rac1, Cdc42, and RhoA. A member of the Dbl family GEFs that regulates GTPase signaling critical for proper cerebellar function. Its expression is developmentally regulated in the murine brain, with pronounced expression in the newborn midbrain and brainstem that wanes with age and maximal expression in adult cerebellar Purkinje neurons. Widely expressed at low levels. More strongly expressed in testis and pancreas. Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. May play a role in cytoskeleton dynamics at the Golgi. Overexpression in NIH3T3 cells induces rearrangements of the actin cytoskeleton including enhanced formation of lamellopodia and fillopodia. Three isoforms of the human protein are produced by alternative splicing. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons.
Protein type: GEFs; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 16q22.1
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity
Biological Process: regulation of Rho protein signal transduction
Disease: Spinocerebellar Ataxia 31; Spinocerebellar Ataxia 4
Research Articles on PLEKHG4
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Product Notes
The PLEKHG4 plekhg4 (Catalog #AAA8242414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLEKHG4 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PLEKHG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the PLEKHG4 plekhg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLEKHG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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