Western Blot (WB)
(Anti- PLN Picoband antibody, MBS178013, Western blottingAll lanes: Anti PLN (MBS178013) at 0.5ug/mlLane 1: Mouse Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: K562 Whole Cell Lysate at 40ugPredicted bind size: 6KDObserved bind size: 18, 24, 36KD )
PLN Polyclonal Antibody | anti-PLN antibody
Anti-PLN Antibody
Western Blot (WB)
(Anti- PLN Picoband antibody, MBS178013, Western blottingAll lanes: Anti PLN (MBS178013) at 0.5ug/mlLane 1: Mouse Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: K562 Whole Cell Lysate at 40ugPredicted bind size: 6KDObserved bind size: 18, 24, 36KD )
Western Blot (WB)
(Anti- PLN Picoband antibody, MBS178013, Western blottingAll lanes: Anti PLN (MBS178013) at 0.5ug/mlLane 1: Mouse Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: K562 Whole Cell Lysate at 40ugPredicted bind size: 6KDObserved bind size: 18, 24, 36KD )
Background: Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. It is observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016]
Uniprot Description
PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.
Protein type: Membrane protein, integral; Inhibitor
Chromosomal Location of Human Ortholog: 6q22.1
Cellular Component: endoplasmic reticulum; membrane; mitochondrial membrane; mitochondrion; perinuclear region of cytoplasm; sarcoplasmic reticulum membrane; vesicle
Molecular Function: ATPase binding; ATPase inhibitor activity; calcium channel regulator activity; enzyme inhibitor activity; identical protein binding; protein binding
Biological Process: blood circulation; calcium ion transport; cardiac muscle development; cytosolic calcium ion homeostasis; negative regulation of ATPase activity; negative regulation of calcium ion transport; negative regulation of catalytic activity; negative regulation of heart rate; Notch signaling pathway; protein homooligomerization; regulation of calcium ion transport; regulation of heart contraction; regulation of the force of heart contraction; response to insulin stimulus; response to testosterone stimulus; response to zinc ion
Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18
Research Articles on PLN
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Product Notes
The PLN pln (Catalog #AAA178013) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-PLN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the PLN pln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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