PLA2G2A sirna

PLA2G2A siRNA (Human)

Cat. Num. AAA8230307

• Gene Names: PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLA2G2A; PLA2G2A siRNA (Human); PLA2B; PLA2L; RASF-A; Phospholipase A2. membrane associated; GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; PLA2G2A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLA2G2A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 144

• Applicable Applications for PLA2G2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLA2G2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLA2G2A sirna

siRNA to inhibit PLA2G2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505849
• NCBI GeneID: 5320
• NCBI Accession #: NP_000291.1
• NCBI GenBank Nucleotide #: NM_000300.3
• UniProt Accession #: P14555; Q6DN24; Q6IBD9; Q9UCD2; A8K5I7
• Molecular Weight: 16,083 Da
• NCBI Official Full Name: phospholipase A2, membrane associated
• NCBI Official Synonym Full Names: phospholipase A2, group IIA (platelets, synovial fluid)
• NCBI Official Symbol: PLA2G2A
• NCBI Official Synonym Symbols: MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• NCBI Protein Information: phospholipase A2, membrane associated
• UniProt Protein Name: Phospholipase A2, membrane associated
• Protein Family: Phospholipase
• UniProt Gene Name: PLA2G2A
• UniProt Synonym Gene Names: PLA2B; PLA2L; RASF-A; NPS-PLA2
• UniProt Entry Name: PA2GA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Secreted, signal peptide; Vesicle; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; Secreted; Phospholipase; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Mitochondrial; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: extracellular space; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; phospholipid binding; calcium ion binding

Biological Process: defense response to Gram-positive bacterium; somatic stem cell maintenance; phosphatidic acid metabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process; negative regulation of epithelial cell proliferation; positive regulation of inflammatory response

Disease: Colorectal Cancer