PLA2G12A sirna

PLA2G12A siRNA (Human)

Cat. Num. AAA8213634

• Gene Names: PLA2G12A; GXII; ROSSY; PLA2G12
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLA2G12A; PLA2G12A siRNA (Human); PLA2G12; Group XIIA secretory phospholipase A2; GXII sPLA2; sPLA2-XII; Phosphatidylcholine 2-acylhydrolase 12A; PLA2G12A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLA2G12A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 189

• Applicable Applications for PLA2G12A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLA2G12A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLA2G12A sirna

siRNA to inhibit PLA2G12A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21361944
• NCBI GeneID: 81579
• NCBI Accession #: NP_110448.2
• NCBI GenBank Nucleotide #: NM_030821.4
• UniProt Accession #: Q9BZM1; Q9BZ89
• Molecular Weight: 21,067 Da
• NCBI Official Full Name: group XIIA secretory phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group XIIA
• NCBI Official Symbol: PLA2G12A
• NCBI Official Synonym Symbols: GXII; ROSSY; PLA2G12
• NCBI Protein Information: group XIIA secretory phospholipase A2
• UniProt Protein Name: Group XIIA secretory phospholipase A2
• Protein Family: Group XIIA secretory phospholipase
• UniProt Gene Name: PLA2G12A
• UniProt Synonym Gene Names: PLA2G12; GXII sPLA2; sPLA2-XII
• UniProt Entry Name: PG12A_HUMAN

NCBI Description

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008]

Uniprot Description

PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family.

Protein type: Secreted; EC 3.1.1.4; Endoplasmic reticulum; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - alpha-linolenic acid; Phospholipase; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Golgi apparatus; endoplasmic reticulum; extracellular region

Molecular Function: calcium-dependent phospholipase A2 activity; calcium ion binding

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process