PIGM sirna

PIGM siRNA (Human)

Cat. Num. AAA8229383

• Gene Names: PIGM; GPI-MT-I
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGM; PIGM siRNA (Human); GPI mannosyltransferase 1; GPI mannosyltransferase I; GPI-MT-I; Phosphatidylinositol-glycan biosynthesis class M protein; PIG-M; PIGM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PIGM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 423

• Applicable Applications for PIGM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIGM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGM sirna

siRNA to inhibit PIGM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21553315
• NCBI GeneID: 93183
• NCBI Accession #: NP_660150.1
• NCBI GenBank Nucleotide #: NM_145167.2
• UniProt Accession #: Q9H3S5
• Molecular Weight: 49,460 Da
• NCBI Official Full Name: GPI mannosyltransferase 1
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class M
• NCBI Official Symbol: PIGM
• NCBI Official Synonym Symbols: GPI-MT-I
• NCBI Protein Information: GPI mannosyltransferase 1
• UniProt Protein Name: GPI mannosyltransferase 1
• Protein Family: Pigment-dispersing hormone
• UniProt Gene Name: PIGM
• UniProt Synonym Gene Names: GPI-MT-I; PIG-M
• UniProt Entry Name: PIGM_HUMAN

NCBI Description

This gene encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008]

Uniprot Description

PIGM: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Defects in PIGM are the cause of glycosylphosphatidylinositol deficiency (GPID). GPID is an autosomal recessive trait that results in a propensity to venous thrombosis and seizures. Deficiency is due to a point mutation in the regulatory sequences of PIGM that disrupts binding of the transcription factor SP1 to its cognate promoter motif, leading to a strong reduction of expression. Belongs to the PIGM family.

Protein type: Membrane protein, multi-pass; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Transferase; Membrane protein, integral; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: mannosyltransferase activity

Biological Process: cellular protein metabolic process; preassembly of GPI anchor in ER membrane; C-terminal protein lipidation; post-translational protein modification

Disease: Glycosylphosphatidylinositol Deficiency