Rabbit anti-Human FOXP1 Polyclonal Antibody | anti-FOXP1 antibody

FOXP1, CT (FOXP1, Forkhead box protein P1) (AP)

Cat. Num. AAA6299960

• Gene Names: FOXP1; QRF1; 12CC4; hFKH1B; HSPC215
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FOXP1; Polyclonal Antibody; CT (FOXP1; Forkhead box protein P1) (AP); Forkhead box protein P1; anti-FOXP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FOXP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-FOXP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 650-677 in the C-terminal region of human FOXP1, UniProt Accession #Q9H334. Species sequence homology: bovine, mouse, rat
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS6110449 in A549, Daudi, Jurkat, MCF-7, NCI-H292 cell line lysates (35ug/lane). This demonstrates the FOXP1 antibody detected the FOXP1 protein (arrow).)

Immunohistochemistry (IHC)

(IHC analysis of MBS6110449 in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MBS6110449 using Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FOXP1 antibody

This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue-and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding-and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s).

Product Categories/Family for anti-FOXP1 antibody

Antibodies; Transcription Factors

References

Johansson, A., et al. Obesity (Silver Spring) 18(4):803-808(2010). Baro, C., et al. Histol. Histopathol. 24(11):1399-1404(2009). Rayoo, M., et al. J. Clin. Pathol. 62(10):896-902(2009). Courts, C., et al. J. Neuropathol. Exp. Neurol. 68(9):972-976(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 348605235
• NCBI GeneID: 27086
• NCBI Accession #: NP_001231745.1
• NCBI GenBank Nucleotide #: NM_001244816.1
• UniProt Accession #: Q9H334; Q8NAN6; Q9BSG9; Q9H332; Q9H333; Q9P0R1; A3QVP8; B3KV70; G5E9V8
• Molecular Weight: 75,317 Da
• NCBI Official Full Name: forkhead box protein P1 isoform 1
• NCBI Official Synonym Full Names: forkhead box P1
• NCBI Official Symbol: FOXP1
• NCBI Official Synonym Symbols: QRF1; 12CC4; hFKH1B; HSPC215
• NCBI Protein Information: forkhead box protein P1; glutamine-rich factor 1; fork head-related protein like B
• UniProt Protein Name: Forkhead box protein P1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP1
• UniProt Entry Name: FOXP1_HUMAN

NCBI Description

This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP1: Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development. Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; androgen receptor binding; sequence-specific DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of smooth muscle cell proliferation; response to lipopolysaccharide; osteoclast differentiation; endothelial cell activation; regulation of transcription from RNA polymerase II promoter; macrophage activation; regulation of monocyte differentiation; monocyte activation; regulation of inflammatory response; regulation of interleukin-1 beta secretion; regulation of tumor necrosis factor production; negative regulation of transcription, DNA-dependent; negative regulation of B cell apoptosis

Disease: Mental Retardation With Language Impairment And With Or Without Autistic Features