Rabbit anti-Human, Mouse Glutathione S-transferase Mu 1 Polyclonal Antibody | anti-GSTM1 antibody

Glutathione S-transferase Mu 1, CT (GST Class-mu 1, GSTM1, GTM1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GST HB Subunit 4, GTH4, GST1, H-B, MGC26563) (Azide free) (HRP)

Cat. Num. AAA6478268

• Gene Names: GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Glutathione S-transferase Mu 1; Polyclonal Antibody; CT (GST Class-mu 1; GSTM1; GTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GST HB Subunit 4; GTH4; GST1; H-B; MGC26563) (Azide free) (HRP); EC=2.5.1.18; anti-GSTM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GSTM1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GSTM1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GSTM1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GSTM1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). GSTM1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GSTM1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane).GSTM1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GSTM1 (arrow) using rabbit polyclonal GSTM1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GSTM1 gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GSTM1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GSTM1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-GSTM1 antibody

Antibodies; GST

References

1. Kostrykina, N.A., et.al., Bull. Exp. Biol. Med. 148(1), 89-93 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 23065544
• NCBI GeneID: 2944
• NCBI Accession #: NP_000552.2
• NCBI GenBank Nucleotide #: NM_000561.3
• UniProt Accession #: P09488; Q5GHG0; Q6FH88; Q8TC98; Q9UC96
• Molecular Weight: 21,253 Da
• NCBI Official Full Name: glutathione S-transferase Mu 1 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase mu 1
• NCBI Official Symbol: GSTM1
• NCBI Official Synonym Symbols: MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• NCBI Protein Information: glutathione S-transferase Mu 1; GST HB subunit 4; GST class-mu 1; S-(hydroxyalkyl)glutathione lyase; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1
• UniProt Protein Name: Glutathione S-transferase Mu 1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM1
• UniProt Synonym Gene Names: GST1
• UniProt Entry Name: GSTM1_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]