PER1 sirna

PER1 siRNA (Human)

Cat. Num. AAA8236570

• Gene Names: PER1; PER; hPER; RIGUI
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PER1; PER1 siRNA (Human); KIAA0482; PER; RIGUI; Period circadian protein homolog 1; hPER1; Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; PER1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PER1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: –

• Applicable Applications for PER1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PER1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PER1 sirna

siRNA to inhibit PER1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194097341
• NCBI GeneID: 5187
• NCBI Accession #: NP_002607.2
• NCBI GenBank Nucleotide #: NM_002616.2
• UniProt Accession #: O15534; B2RPA8; B4DI49; D3DTR3
• Molecular Weight: – Da
• NCBI Official Full Name: period circadian protein homolog 1
• NCBI Official Synonym Full Names: period circadian clock 1
• NCBI Official Symbol: PER1
• NCBI Official Synonym Symbols: PER; hPER; RIGUI
• NCBI Protein Information: period circadian protein homolog 1
• UniProt Protein Name: Period circadian protein homolog 1
• Protein Family: Protein
• UniProt Gene Name: PER1
• UniProt Synonym Gene Names: KIAA0482; PER; RIGUI; hPER1
• UniProt Entry Name: PER1_HUMAN

NCBI Description

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014]

Uniprot Description

PER1: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Can bind heme. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; nucleus

Molecular Function: signal transducer activity; chromatin DNA binding; ubiquitin protein ligase binding; transcription factor binding; kinase binding

Biological Process: circadian rhythm; negative regulation of JNK cascade; response to cAMP; transcription, DNA-dependent; negative regulation of I-kappaB kinase/NF-kappaB cascade; regulation of sodium ion transport; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; signal transduction; entrainment of circadian clock by photoperiod; positive regulation of transcription from RNA polymerase II promoter; entrainment of circadian clock; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; regulation of hair cycle