PASK sirna

PASK siRNA (Human)

Cat. Num. AAA8232916

• Gene Names: PASK; STK37; PASKIN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PASK; PASK siRNA (Human); KIAA0135; PAS domain-containing serine/threonine-protein kinase; PAS-kinase; PASKIN; hPASK; PASK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PASK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1330

• Applicable Applications for PASK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PASK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PASK sirna

siRNA to inhibit PASK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 355477262
• NCBI GeneID: 23178
• NCBI Accession #: NP_001239048.1
• NCBI GenBank Nucleotide #: NM_001252119.1
• UniProt Accession #: Q96RG2; Q05BE4; Q68DY3; Q6GSJ5; Q86XH6; Q99763; Q9UFR7; G5E9F1
• Molecular Weight: 123,048 Da
• NCBI Official Full Name: PAS domain-containing serine/threonine-protein kinase isoform 1
• NCBI Official Synonym Full Names: PAS domain containing serine/threonine kinase
• NCBI Official Symbol: PASK
• NCBI Official Synonym Symbols: STK37; PASKIN
• NCBI Protein Information: PAS domain-containing serine/threonine-protein kinase
• UniProt Protein Name: PAS domain-containing serine/threonine-protein kinase
• Protein Family: PAS domain-containing serine/threonine-protein kinase
• UniProt Gene Name: PASK
• UniProt Synonym Gene Names: KIAA0135; PAS-kinase; PASKIN; hPASK
• UniProt Entry Name: PASK_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

PASK: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation. Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails. Protein kinase activity is inhibited by the first PAS domain: binding of an unidentified ligand desinhibits the protein kinase activity. May be activated by autophosphorylation on Thr-1161 and Thr-1165 (PubMed:11459942). The activating role of autophosphorylation at Thr-1161 is unclear: according to a report, autophosphorylation at Thr-1161 does not play a major role in activation (PubMed:20943661). Autophosphorylation is enhanced upon phosphatidylinositol monophosphate (phosphatidylinositol 4-phosphate) binding and inhibited upon phosphatidylinositol bi- and tri-phosphate binding. In contrast, phosphorylation of target proteins is inhibited upon all phosphatidylinositol-binding (phosphatidylinositol mono- bi- and tri-phosphate). Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; EC 2.7.11.1; CAMK group; CAMKL family; PASK subfamily

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: Golgi apparatus; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; phosphoinositide binding; ATP binding

Biological Process: regulation of respiratory gaseous exchange; positive regulation of translation; protein amino acid autophosphorylation; signal transduction; protein amino acid phosphorylation; negative regulation of glycogen biosynthetic process