Rabbit PASK Polyclonal Antibody | anti-PASK antibody

PASK Rabbit Polyclonal

Cat. Num. AAA7602398

• Gene Names: PASK; STK37; PASKIN
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: PASK; Polyclonal Antibody; PASK Rabbit Polyclonal; anti-PASK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 1330

• Applicable Applications for anti-PASK antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: PAS domain containing serine/threonine kinase
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue slide using MBS7602398 (PASK Antibody) at dilution of 1:50)

Western Blot (WB)

(K-562 cells were subjected to SDS PAGE followed by western blot with MBS7602398 (PASK antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 355477262
• NCBI GeneID: 23178
• NCBI Accession #: NP_001239048.1
• NCBI GenBank Nucleotide #: NM_001252119.1
• Molecular Weight: 123,048 Da
• NCBI Official Full Name: PAS domain-containing serine/threonine-protein kinase isoform 1
• NCBI Official Synonym Full Names: PAS domain containing serine/threonine kinase
• NCBI Official Symbol: PASK
• NCBI Official Synonym Symbols: STK37; PASKIN
• NCBI Protein Information: PAS domain-containing serine/threonine-protein kinase
• UniProt Protein Name: PAS domain-containing serine/threonine-protein kinase
• Protein Family: PAS domain-containing serine/threonine-protein kinase
• UniProt Gene Name: PASK
• UniProt Synonym Gene Names: KIAA0135; PAS-kinase; PASKIN; hPASK
• UniProt Entry Name: PASK_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

PASK: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation. Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails. Protein kinase activity is inhibited by the first PAS domain: binding of an unidentified ligand desinhibits the protein kinase activity. May be activated by autophosphorylation on Thr-1161 and Thr-1165 (PubMed:11459942). The activating role of autophosphorylation at Thr-1161 is unclear: according to a report, autophosphorylation at Thr-1161 does not play a major role in activation (PubMed:20943661). Autophosphorylation is enhanced upon phosphatidylinositol monophosphate (phosphatidylinositol 4-phosphate) binding and inhibited upon phosphatidylinositol bi- and tri-phosphate binding. In contrast, phosphorylation of target proteins is inhibited upon all phosphatidylinositol-binding (phosphatidylinositol mono- bi- and tri-phosphate). Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; CAMKL family; PASK subfamily

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; Golgi apparatus; nucleus

Molecular Function: ATP binding; phosphoinositide binding; protein binding; protein serine/threonine kinase activity

Biological Process: negative regulation of glycogen biosynthetic process; positive regulation of translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of respiratory gaseous exchange