Typical Testing Data/Standard Curve (for reference only)
Human S100 Calcium Binding Protein A9 ELISA Kit | S100A9 elisa kit
Human S100 Calcium Binding Protein A9 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: S100A9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A9. Standards or samples are then added to the microtiter plate wells and S100A9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100A9 are added to each well to "sandwich" the S100A9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A9 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: extracellular space; cytoskeleton; extracellular region; plasma membrane; nucleus; cytosol
Molecular Function: arachidonic acid binding; antioxidant activity; protein binding; signal transducer activity; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding
Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; chemokine production; cytokine production; positive regulation of peptide secretion; response to lipopolysaccharide; signal transduction; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; cell-cell signaling; response to zinc ion; defense response to bacterium; actin cytoskeleton reorganization; autophagy; innate immune response; regulation of integrin biosynthetic process; inflammatory response; defense response to fungus; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis
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Product Notes
The Human S100A9 s100a9 (Catalog #AAA721450) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA721450 ELISA Kit recognizes Human S100A9. It is sometimes possible for the material contained within the vial of "S100 Calcium Binding Protein A9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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