PACRG sirna

PACRG siRNA (Human)

Cat. Num. AAA8234659

• Gene Names: PACRG; GLUP; PARK2CRG; HAK005771
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PACRG; PACRG siRNA (Human); GLUP; Parkin coregulated gene protein; Molecular chaperone/chaperonin-binding protein; PARK2 coregulated gene protein; PACRG sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PACRG siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 257

• Applicable Applications for PACRG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PACRG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PACRG sirna

siRNA to inhibit PACRG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 122939202
• NCBI GeneID: 135138
• NCBI Accession #: NP_001073847.1
• NCBI GenBank Nucleotide #: NM_001080378.1
• UniProt Accession #: Q96M98; Q6IMB8; Q8IZM1; Q8NHP5; Q9H1V9; E1P5B5
• Molecular Weight: 29,312 Da
• NCBI Official Full Name: parkin coregulated gene protein isoform 2
• NCBI Official Synonym Full Names: PARK2 co-regulated
• NCBI Official Symbol: PACRG
• NCBI Official Synonym Symbols: GLUP; PARK2CRG; HAK005771
• NCBI Protein Information: parkin coregulated gene protein
• UniProt Protein Name: Parkin coregulated gene protein
• Protein Family: PACRG-like protein
• UniProt Gene Name: PACRG
• UniProt Synonym Gene Names: GLUP
• UniProt Entry Name: PACRG_HUMAN

NCBI Description

This gene encodes a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PACRG: Suppresses cell death induced by accumulation of unfolded Pael receptor (Pael-R, a substrate of Parkin). Facilitates the formation of inclusions consisting of Pael-R, molecular chaperones, protein degradation molecules and itself when proteasome is inhibited. May play an important role in the formation of Lewy bodies and protection of dopaminergic neurons against Parkinson disease. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: neuron projection; mitochondrion; nucleus; cytosol; vesicle

Molecular Function: G-protein-coupled receptor binding; ubiquitin protein ligase binding; heat shock protein binding; chaperone binding; beta-tubulin binding; Hsp70 protein binding; Hsp90 protein binding; actin binding; alpha-tubulin binding

Biological Process: spermatid development

Disease: Leprosy, Susceptibility To, 2