ORC1 sirna

ORC1 siRNA (Human)

Cat. Num. AAA8228935

• Gene Names: ORC1; ORC1L; PARC1; HSORC1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ORC1; ORC1 siRNA (Human); ORC1L; PARC1; Origin recognition complex subunit 1; Replication control protein 1; ORC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ORC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 861

• Applicable Applications for ORC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ORC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ORC1 sirna

siRNA to inhibit ORC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 299890793
• NCBI GeneID: 4998
• NCBI Accession #: NP_001177747.1
• NCBI GenBank Nucleotide #: NM_001190818.1
• UniProt Accession #: Q13415; Q13471; Q5T0F5; D3DQ34
• Molecular Weight: 97,350 Da
• NCBI Official Full Name: origin recognition complex subunit 1 isoform 1
• NCBI Official Synonym Full Names: origin recognition complex, subunit 1
• NCBI Official Symbol: ORC1
• NCBI Official Synonym Symbols: ORC1L; PARC1; HSORC1
• NCBI Protein Information: origin recognition complex subunit 1
• UniProt Protein Name: Origin recognition complex subunit 1
• Protein Family: Origin recognition complex
• UniProt Gene Name: ORC1
• UniProt Synonym Gene Names: ORC1L; PARC1
• UniProt Entry Name: ORC1_HUMAN

NCBI Description

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

ORC1L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Belongs to the ORC1 family.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nuclear origin of replication recognition complex; nucleus; cytosol; origin recognition complex

Molecular Function: protein binding; DNA binding; chromatin binding; ATP binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 1