Western Blot (WB)
(Western Blot analysis of 3T3 cells using ORC1 Polyclonal Antibody)
Rabbit anti-Human ORC1 Polyclonal Antibody | anti-ORC1 antibody
ORC1 Polyclonal Antibody
ELISA: 1:5000
Not yet tested in other applications.
Western Blot (WB)
(Western Blot analysis of 3T3 cells using ORC1 Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of 3T3 cells using ORC1 Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of NIH-3T3 HELA 293 cells using ORC1 Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of NIH-3T3 HELA 293 cells using ORC1 Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Uniprot Description
ORC1L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Belongs to the ORC1 family.
Protein type: Motility/polarity/chemotaxis; DNA-binding
Chromosomal Location of Human Ortholog: 1p32
Cellular Component: nucleoplasm; cytoplasm; nucleolus; plasma membrane; nuclear origin of replication recognition complex; nucleus; cytosol; origin recognition complex
Molecular Function: protein binding; DNA binding; chromatin binding; ATP binding
Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
Disease: Meier-gorlin Syndrome 1
Research Articles on ORC1
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Product Notes
The ORC1 orc1 (Catalog #AAA9400966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ORC1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ORC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Western Blot: 1:500 - 1:2000 ELISA: 1:5000 Not yet tested in other applications. Researchers should empirically determine the suitability of the ORC1 orc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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