NXT1 sirna

NXT1 siRNA (Human)

Cat. Num. AAA8203438

• Gene Names: NXT1; P15; MTR2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NXT1; NXT1 siRNA (Human); NTF2-related export protein 1; Protein p15; NXT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NXT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 140

• Applicable Applications for NXT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NXT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NXT1 sirna

siRNA to inhibit NXT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7019471
• NCBI GeneID: 29107
• NCBI Accession #: NP_037380.1
• NCBI GenBank Nucleotide #: NM_013248.2
• UniProt Accession #: Q9UKK6
• Molecular Weight: 15,847 Da
• NCBI Official Full Name: NTF2-related export protein 1
• NCBI Official Synonym Full Names: nuclear transport factor 2-like export factor 1
• NCBI Official Symbol: NXT1
• NCBI Official Synonym Symbols: P15; MTR2
• NCBI Protein Information: NTF2-related export protein 1
• UniProt Protein Name: NTF2-related export protein 1
• Protein Family: NTF2-related export protein
• UniProt Gene Name: NXT1
• UniProt Entry Name: NXT1_HUMAN

NCBI Description

The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NXT1: Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: cytoplasm; nuclear pore; nuclear speck

Molecular Function: protein binding; Ran GTPase binding

Biological Process: RNA export from nucleus; protein export from nucleus