Western Blot (WB)
Rabbit anti-Human PFKFB3 Polyclonal Antibody | anti-PFKFB3 antibody
Anti-PFKFB3 Antibody
Gene Names
PFKFB3; PFK2; IPFK2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
PFKFB3; Polyclonal Antibody; Anti-PFKFB3 Antibody; anti-PFKFB3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
4497
Applicable Applications for anti-PFKFB3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Antibody Type
Primary antibody
Immunogen
Raised against a recombinate human PFKFB3 protein 55-307aa (BC040482).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Western Blot (WB)
Western Blot (WB)
Immunohistochemistry (IHC) #2
Immunohistochemistry (IHC) #2
Related Product Information for anti-PFKFB3 antibody
Rabbit Polyclonal Antibody
References
Tudzarova,S., Colombo,S.L., Stoeber,K., et al. Two ubiquitin ligases, APC/C-Cdh1 and SKP1-CUL1-F (SCF)-beta-TrCP, sequentially regulate glycolysis during the cell cycle. Proc. Natl. Acad. Sci. U.S.A. 108 (13), 5278-5283 (2011)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,602 Da
NCBI Official Full Name
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3, mRNA
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
NCBI Official Symbol
PFKFB3
NCBI Official Synonym Symbols
PFK2; IPFK2
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase; 6PF-2-K/Fru-2,6-P2ase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; PFK/FBPase 3; fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase; iPFK-2; inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase; renal carcinoma antigen NY-REN-56
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Protein Family
UniProt Gene Name
PFKFB3
UniProt Entry Name
F263_HUMAN
Uniprot Description
PFKFB3: Synthesis and degradation of fructose 2,6-bisphosphate. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.46; Carbohydrate Metabolism - fructose and mannose; Kinase, other; Phosphatase (non-protein); EC 2.7.1.105; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10p15.1
Cellular Component: nucleoplasm; cytosol
Molecular Function: fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; ATP binding
Biological Process: glycolysis; dephosphorylation; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; brain development; fructose 2,6-bisphosphate metabolic process; fructose metabolic process
Research Articles on PFKFB3
Similar Products
Product Notes
The PFKFB3 pfkfb3 (Catalog #AAA416646) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PFKFB3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA titer: 1:20,000-1:80,000 Western blot analysis: 1:1,000-1:2,000 Immunohistochemistry: 1:100-1:500 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the PFKFB3 pfkfb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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