NUP85 sirna

NUP85 siRNA (Human)

Cat. Num. AAA8217961

• Gene Names: NUP85; Nup75; FROUNT
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUP85; NUP85 siRNA (Human); NUP75; PCNT1; Nuclear pore complex protein Nup85; 85 kDa nucleoporin; FROUNT; Nucleoporin Nup75; Nucleoporin Nup85; Pericentrin-1; NUP85 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NUP85 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 610

• Applicable Applications for NUP85 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NUP85 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUP85 sirna

siRNA to inhibit NUP85 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 740086933
• NCBI GeneID: 79902
• NCBI Accession #: NP_001290205.1
• NCBI GenBank Nucleotide #: NM_001303276.1
• UniProt Accession #: Q9BW27; Q8NDI4; Q9H9U1; B4DMQ3; B4DPW1
• Molecular Weight: 69,800 Da
• NCBI Official Full Name: nuclear pore complex protein Nup85 isoform 2
• NCBI Official Synonym Full Names: nucleoporin 85kDa
• NCBI Official Symbol: NUP85
• NCBI Official Synonym Symbols: Nup75; FROUNT
• NCBI Protein Information: nuclear pore complex protein Nup85
• UniProt Protein Name: Nuclear pore complex protein Nup85
• Protein Family: Nucleoporin
• UniProt Gene Name: NUP85
• UniProt Synonym Gene Names: NUP75; PCNT1
• UniProt Entry Name: NUP85_HUMAN

NCBI Description

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

NUP85: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. Belongs to the nucleoporin Nup85 family.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: kinetochore; nuclear membrane; membrane; nuclear envelope; spindle; cytosol

Molecular Function: protein binding

Biological Process: lamellipodium biogenesis; mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; macrophage chemotaxis; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport